C57BL/6N-Hmgcrem1Lclem/Kctt
| Status | Available to order |
| EMMA ID | EM:14795 |
| International strain name | C57BL/6N-Hmgcrem1Lclem/Kctt |
| Alternative name | C57BL/6N-Hmgcrem1Lclem/Lclem |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Hmgcrem1Lclem |
| Gene/Transgene symbol | Hmgcr |
Information from provider
| Provider | Helgi Schiöth |
| Provider affiliation | Department of Surgical Sciences, Functional Pharmacology and Neuroscience, Uppsala University |
| Genetic information | LoxP sites have been inserted around exon 5 of the Hmgcr gene by CRISPR/Cas9 mediated genome engineering. CRISPR technology details: Cas9 protein (from New England Biolabs) and gRNA were co-injected into fertilized eggs, therefore Cas9 did not integrate in the mouse genome. |
| Phenotypic information | Homozygous:The mice have no pathological phenotype, since the mutation is conditional.Heterozygous:The mice have no pathological phenotype, since the mutation is conditional. |
| Breeding history | Heterozygous founders were bred with C57BL/6NTac wild-type mice to increase the colony. After this, the heterozygous mice were bred to homozigosity and inbred for 4 generations before conservation. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | homozygous 0 |