Sprague Dawley rat Crl:CD(SD)-KO(Gnal*135)44-178Hpng/Hpng

Status	Available to order
EMMA ID	EM:14799
Citation information	EM:14799
International strain name	Sprague Dawley rat Crl:CD(SD)-KO(Gnal*135)44-178Hpng/Hpng
Alternative name	Sprague Dawley rat Crl:CD(SD)-KO(Gnal*135)44-178Hpng/Hpng
Strain type	Endonuclease-mediated
Allele/Transgene symbol	rat Gnal^del44-178
Gene/Transgene symbol	rat Gnal

Information from provider

Provider	Huu Phuc Nguyen
Provider affiliation	Institute od Medical Genetics and Applied Genomics, University of Tuebingen
Genetic information	Crl:CD(SD)-KO(Gnal*135)44-178Hpng/Hpng rats were generated by CRISPR/Cas9 technology. A deletion of 135 base pairs, corresponding to position 44-178, downstream of the translation initiation start ATG of the Gnal splicing variant 2, results in a deletion mutation. CRISPR technology details: self-synthesized Cas9 mRNA using px 330 vector, applying T7 transcription kit followed by Poly-Adenylation and Capping. No sequencing performed, whether Cas9 has been inserted into the rat genome is unknown.
Phenotypic information	Homozygous: No homozygous was generated Heterozygous: No characterization of phenotype
Breeding history	Crl:CD(SD)-KO(Gnal*135)44-178Hpng/Hpng is an outbred strain, the status now is about 16 generations.
References	None available
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous rat Sprague-Dawley males, wild-type rat Sprague-Dawley females
Stage of embryos	2-cell

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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