IMPC phenotypes (gene matching)
STOCK Daglatm1.2Zim/Orl
| Status | Under development - register interest |
| EMMA ID | EM:14883 |
| Citation information | RRID:IMSR_EM:14883 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Daglatm1.2Zim/Orl |
| Alternative name | B6.cg Dagla tm1.2Zim |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Daglatm1.2Zim |
| Gene/Transgene symbol | Dagla |
Information from provider
| Provider | Andreas Zimmer |
| Provider affiliation | Institut für Molekulare Pychiatrie |
| Genetic information | The murine Dagla gene is located at chromosome 19 and comprises 20 exons. In the Dagla-tm1.2Zim (Dagla knock-out) line the exon 1 was removed by cre recombinase-mediated excision (the floxed strain used is EMMA strain EM:14881). Depiction of the cloning and knock-out strategy is shown at Fig. 1 in Jenniches et al., Biol. Psychiatry, 2015. |
| Phenotypic information | Homozygous:The Dagla-tm1.2Zim homozygous knock-out line is epilepsy prone, spontaneous epileptic seizures are observed especially in stress situation. The body weight of the mice is lower than in wild-type littermates from a heterozygous breeding. The behavioral changes induced by Dagla deletion include a reduced exploration of the central area of the open field, a maternal neglect behavior, a fear extinction deficit, increased behavioral despair, increased anxiety-related behaviors in the light/dark box, and reduced hippocampal neurogenesis. Heterozygous:Heterozygous mice were not tested. |
| Breeding history | 10 generations on C57BL/6J. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | homozygous C57BL/6J males |
Disease and phenotype information
MGI phenotypes (gene matching)
- decreased body weight / MGI
- abnormal lipid level / MGI
- absent long term depression / MGI
- abnormal CNS synaptic transmission / MGI
- abnormal excitatory postsynaptic currents / MGI
- abnormal inhibitory postsynaptic currents / MGI
- increased single cell response threshold / MGI
- abnormal nervous system morphology / MGI
- decreased neuronal precursor cell number / MGI
- abnormal neuron proliferation / MGI
Literature references
- Anxiety, Stress, and Fear Response in Mice With Reduced Endocannabinoid Levels.;Jenniches Imke, Ternes Svenja, Albayram Onder, Otte David M, Bach Karsten, Bindila Laura, Michel Kerstin, Lutz Beat, Bilkei-Gorzo Andras, Zimmer Andreas, ;2016;Biological psychiatry;79;858-868; 25981172