C57BL/6-Mpc1tm-(tetO)Itl/H
| Status | Available to order |
| EMMA ID | EM:14916 |
| International strain name | C57BL/6-Mpc1tm-(tetO)Itl/H |
| Alternative name | MPC1 tetO KI |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Mpc1-FAST |
| Gene/Transgene symbol | Mpc1 |
Information from provider
| Provider | Philip Eaton |
| Provider affiliation | Queen Mary University |
| Genetic information | Targeted Mpc1 FAST (Flexible Accelerated STOP Tetracycline Operator (tetO) knock-in) mouse line was generated by Ingenious Targeting Laboratory (iTL) technologies (Ronkonkoma, New York). Briefly, vectors that contained a loxP-tetO (PTre3G promoter) sequence were inserted immediately upstream of the endogenous ATG initiation site in exon 1 of the mitochondrial pyruvate carrier 1 (Mpc1) gene sequence. A single gene targeting event yields two distinct knock-in mice: STOP-tetO and tetO knockin that permits generation of multiple strains with variable expression patterns: 1) knockout, 2) cre-mediated rescue, 3) tetracycline-controlled transcriptional activator (tTA)-mediated misexpression, 4) tetracycline-controlled transcriptional activator (tTA)-mediated overexpression, and 5) tetracycline-controlled transcriptional silencer (tTS)-mediated conditional knockout/knockdown. Using the FAST system, multiple gain-of-function and loss-of-function strains can therefore be generated on a time scale not previously achievable. |
| Phenotypic information | Homozygous:Phenotypic effects only shown when crossed to mice carrying the cre/loxP and the tetracycline on/off systems.Heterozygous:Phenotypic effects only shown when crossed to mice carrying the cre/loxP and the tetracycline on/off systems. |
| Breeding history | These mice were backcrossed more than 10 times to C57BL/6NJ over the last 5 years. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Mitochondrial pyruvate carrier abundance mediates pathological cardiac hypertrophy.;Fernandez-Caggiano Mariana, Kamynina Alisa, Francois Asvi A, Prysyazhna Oleksandra, Eykyn Thomas R, Krasemann Susanne, Crespo-Leiro Maria G, Vieites Maria Garcia, Bianchi Katiuscia, Morales Valle, Domenech Nieves, Eaton Philip, ;2020;Nature metabolism;2;1223-1231; 33106688