IMPC phenotypes (gene matching)
B6N(Cg)-Palmtm1.2Kili/Ieg
| Status | Available to order |
| EMMA ID | EM:14921 |
| Citation information | RRID:IMSR_EM:14921 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6N(Cg)-Palmtm1.2Kili/Ieg |
| Alternative name | Palm-tm1.2Kili (official allele design., MGI:6883588) lab. design.: Palm1k (constit. KO) |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Palmtm1.2Kili |
| Gene/Transgene symbol | Palm |
Information from provider
| Provider | Manfred Kilimann |
| Provider affiliation | Molecular Neurobiology, MPI for Multidisciplinary Sciences |
| Genetic information | Palm gene constitutive knock-out mice were constructed by TaconicArtemis (Köln, Germany) by cre recombinase-mediated deletion of exon 5 in the germline. The original, conditional allele before cre recombination was Palmtm1.1Kili, MGI:6883582 (EMMA strain EM:14920). Deletion of exon 5 causes a frame-shift. Western blot analysis demonstrated that this is a null mutation (protein expression undetectable, detection limit |
| Phenotypic information | Homozygous:Homozygous mice are apparently unaffected and fertile. Phenotyping at the German Mouse Clinic detected a few slight anomalies of unclear significance. In-depth research detected anomalies of tumor lymphangiogenesis.Heterozygous:No phenotypes. |
| Breeding history | The mutation was generated in a C57BL/6N ES cell line and mice were subsequently backcrossed to C57BL/6N mice for more than 5 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | homozygous C57BL/6N males |
Disease and phenotype information
Literature references
- Paralemmin-1 is expressed in lymphatic endothelial cells and modulates cell migration, cell maturation and tumor lymphangiogenesis.;Albrecht Imke, Bieri Raphael, Leu Angela, Granacher Philipp, Hagmann Jörg, Kilimann Manfred W, Christofori Gerhard, ;2013;Angiogenesis;16;795-807; 23709172
- Neocortical Expansion Due to Increased Proliferation of Basal Progenitors Is Linked to Changes in Their Morphology.;Kalebic Nereo, Gilardi Carlotta, Stepien Barbara, Wilsch-Bräuninger Michaela, Long Katherine R, Namba Takashi, Florio Marta, Langen Barbara, Lombardot Benoit, Shevchenko Anna, Kilimann Manfred W, Kawasaki Hiroshi, Wimberger Pauline, Huttner Wieland B, ;2019;Cell stem cell;24;535-550.e9; 30905618