C57BL/6J-Ptprcem1Jmar/Orl

Status

Available to order

EMMA IDEM:15082
Citation informationRRID:IMSR_EM:15082 

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International strain nameC57BL/6J-Ptprcem1Jmar/Orl
Alternative nameB6.Ptprc943K/E/Jmar
Strain typeEndonuclease-mediated
Allele/Transgene symbolPtprcem1Jmar
Gene/Transgene symbolPtprc

Information from provider

ProviderJacqueline Marvel
Provider affiliationCIRI INSERM
Genetic informationC57BL/6J isogenic mouse strain that contains a mutation in the aa 302 (A904G to K302E) of the Ptprc gene leading to an amino acid change (K/E). This mouse line, generated by CRISPR genome editing, expresses the Ptprca (CD45.1) rather than the Ptprcb (CD45.2) isoform normally expressed by C57BL/6J mice. The line was generated using CRISPR/Cas9 genome editing. Guide RNA was selected to target exon 10 of the Ptprc gene on chromosome 1. Donor DNAs was designed in order to replace a lysine into glutamic acid (nucleotide sequence at aa 302: AAA to GAA). Four silent mutations were introduced in order to prevent cutting of the mutated allele by the Cas9 protein.
Phenotypic informationHomozygous:
Hematopoietic cells of the mouse express the CD45.1 epitope instead on the CD45.2 epitope.

Heterozygous:
Hematopoietic cells of the mouse express both the CD45.1 epitope and the CD45.2 epitope.
Breeding historyDNAs of F0 mosaic mice were sequenced to screen for the mutation. Correctly mutated pups were then bred to C57BL/6J mice for homozygous mouse strain generation. The B6.Ptprc302K/E/Jmar colony was backcrossed to C57BL/6J twice.
References
  • Generation of a C57BL/6J mouse strain expressing the CD45.1 epitope to improve hematopoietic stem cell engraftment and adoptive cell transfer experiments.;Laubreton Daphné, Djebali Sophia, Angleraux Céline, Chain Benny, Dubois Maxence, Henry Farida, Leverrier Yann, Teixeira Marie, Markossian Suzy, Marvel Jacqueline, ;2023;Lab animal;52;324-331; 38017180
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France
Animals used for archivinghomozygous C57BL/6J males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (gene matching)
  • abnormal long bone metaphysis morphology / MGI
  • abnormal leukocyte cell number / MGI
  • increased neutrophil cell number / MGI
  • decreased leukocyte cell number / MGI
  • extramedullary hematopoiesis / MGI
  • abnormal erythropoiesis / MGI
  • enlarged spleen / MGI
  • spleen hyperplasia / MGI
  • enlarged lymph nodes / MGI
  • decreased thymocyte number / MGI
  • abnormal immune system cell morphology / MGI
  • abnormal myelination / MGI
  • abnormal oligodendrocyte morphology / MGI
  • abnormal osteoclast physiology / MGI
  • abnormal digestion / MGI
  • abnormal immune system physiology / MGI
  • abnormal humoral immune response / MGI
  • thymus hypoplasia / MGI
  • arrested T cell differentiation / MGI
  • abnormal T cell activation / MGI
  • liver inflammation / MGI
  • lung inflammation / MGI
  • premature death / MGI
  • abnormal T cell differentiation / MGI
  • no abnormal phenotype detected / MGI
  • abnormal bone marrow cell morphology/development / MGI
  • abnormal lymphopoiesis / MGI
  • abnormal double-positive T cell morphology / MGI
  • increased susceptibility to viral infection / MGI
  • abnormal T cell physiology / MGI
  • abnormal B cell number / MGI
  • increased IgA level / MGI
  • abnormal immune system organ morphology / MGI
  • glomerulonephritis / MGI
  • increased urine protein level / MGI
  • abnormal cytokine secretion / MGI
  • no phenotypic analysis / MGI
  • kidney failure / MGI
  • oliguria / MGI
  • abnormal thymocyte activation / MGI
  • abnormal T cell subpopulation ratio / MGI
  • increased anti-double stranded DNA antibody level / MGI
  • abnormal T cell selection / MGI
  • abnormal positive T cell selection / MGI
  • increased B-1 B cell number / MGI
  • abnormal osteoclast morphology / MGI
  • increased B cell number / MGI
  • increased T cell number / MGI
  • decreased B cell number / MGI
  • decreased T cell number / MGI
  • abnormal response to infection / MGI
  • diarrhea / MGI
  • decreased cytotoxic T cell cytolysis / MGI
  • increased double-negative T cell number / MGI
  • decreased double-positive T cell number / MGI
  • decreased B cell proliferation / MGI
  • decreased T cell proliferation / MGI
  • increased susceptibility to autoimmune disorder / MGI
  • immune system phenotype / MGI
  • hematopoietic system phenotype / MGI
  • decreased susceptibility to type I hypersensitivity reaction / MGI
  • CNS inflammation / MGI
  • increased T cell apoptosis / MGI
  • abnormal T cell morphology / MGI
  • increased NK cell number / MGI
  • abnormal memory T cell number / MGI
  • increased memory T cell number / MGI
  • decreased memory T cell number / MGI
  • absent T cells / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • abnormal CD4-positive T cell differentiation / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • abnormal CD8-positive, alpha-beta T cell differentiation / MGI
  • increased single-positive T cell number / MGI
  • decreased single-positive T cell number / MGI
  • increased plasma cell number / MGI
  • lymph node hyperplasia / MGI
  • abnormal granulocyte differentiation / MGI
  • increased dendritic cell number / MGI
  • decreased B-1a cell number / MGI
  • increased B-1b cell number / MGI
  • decreased B-1b cell number / MGI
  • decreased follicular B cell number / MGI
  • increased transitional stage B cell number / MGI
  • abnormal follicular B cell physiology / MGI
  • decreased B-2 B cell number / MGI
  • decreased mature B cell number / MGI
  • abnormal B cell activation / MGI
  • abnormal leukocyte morphology / MGI
  • decreased gamma-delta intraepithelial T cell number / MGI
  • abnormal CD4-positive, alpha-beta intraepithelial T cell morphology / MGI
  • increased spleen red pulp amount / MGI
  • increased spleen white pulp amount / MGI
  • increased IgG2a level / MGI
  • increased plasmacytoid dendritic cell number / MGI
  • abnormal chemokine secretion / MGI
  • decreased memory B cell number / MGI
  • decreased mast cell degranulation / MGI
  • abnormal intraepithelial T cell morphology / MGI
  • abnormal intraepithelial T cell number / MGI
  • decreased oligodendrocyte number / MGI
  • increased DN3 thymocyte number / MGI
  • decreased DN4 thymocyte number / MGI
  • abnormal NK cell physiology / MGI
  • mortality/aging / MGI
  • decreased CD4-positive, alpha-beta memory T cell number / MGI
  • decreased CD8-positive, alpha-beta memory T cell number / MGI
  • lethality at weaning, incomplete penetrance / MGI
  • increased alpha-beta T cell number / MGI
  • decreased CD8-positive, naive alpha-beta T cell number / MGI

Literature references

  • Generation of a C57BL/6J mouse strain expressing the CD45.1 epitope to improve hematopoietic stem cell engraftment and adoptive cell transfer experiments.;Laubreton Daphné, Djebali Sophia, Angleraux Céline, Chain Benny, Dubois Maxence, Henry Farida, Leverrier Yann, Teixeira Marie, Markossian Suzy, Marvel Jacqueline, ;2023;Lab animal;52;324-331; 38017180

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

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Practical information

Genotyping protocol

Example health report
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Legally binding conditions for the transfer

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