STOCK Npm1floxed/Cnrm
| Status | Available to order |
| EMMA ID | EM:15106 |
| International strain name | STOCK Npm1floxed/Cnrm |
| Alternative name | C57/BL6 NPM1/KO |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Npm1flox |
| Gene/Transgene symbol | Npm1 |
Information from provider
| Provider | Paolo Sportoletti |
| Provider affiliation | Department of Medicine and Surgery, University of Perugia |
| Genetic information | Based on the Npm1 gene organisation and the predicted functional protein structure, a targeting strategy was designed, resulting in the conditional deletion of exon 1. This strategy results in the deletion of 58 bp coding sequences encoding for the N-terminal 1 methionine-rich region and the start-ATG codon present in exon 1, and should thus result in the absence of any expression for the Npm1 gene and therefore of any known isoform. |
| Phenotypic information | Homozygous:The nucleophosmin (Npm1) gene encodes for a nucleolar phosphoprotein abundantly and ubiquitously expressed. NPM1 has proved to be a multifunctional protein involved in many cellular activities. NPM1 oligomerizes and is part of a high molecular weight complex physically interacting with many cellular proteins including p53. Splice variants of Npm1 were shown to have different level of mRNA and protein expression. NPM is implicated in human tumorigenesis, is frequently overexpressed in solid tumors of a diverse histological origin, and genetic alteration of NPM frequently occurs in hematopoietic tumors (chromosomal translocation, mutations). The Npm1 gene is a partner in chromosomal translocations of leukaemia and lymphomas resulting in fusion proteins containing only the NPM N-terminal region. In addition, it has been shown that one-third of acute myeloid leukaemia (AML) is characterized by the aberrant cytoplasmic localisation of NPM (isoform 1) due to mutations leading to a frame shift in the last coding exon. To allow the role of the Npm1 gene to be further investigated in vivo, a conditional knock-out model has been generated. This model allows the tissue-restricted inactivation of nucleophosmin.Heterozygous:. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | yes |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |