B6;129-Bcar1floxed/Cnrm
| Status | Under development - register interest |
| EMMA ID | EM:15110 |
| International strain name | B6;129-Bcar1floxed/Cnrm |
| Alternative name | bcar1 floxed |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Bcar1flox |
| Gene/Transgene symbol | Bcar1 |
Information from provider
| Provider | Sara Cabodi |
| Provider affiliation | University of Torino |
| Genetic information | Floxed mice for the Bcar1 gene were generated by ES recombination with a construct carrying loxP sites flanking exon 5 and 6 of Bcar1 gene and the neomycin cassette, as reported in Camacho Leal MDP et al., 2018 (https://doi.org/10.1186/s12964-018-0289-z). The selection cassette was removed by crossing the mice with a flipper (FLPe) strain. When cre recombinase is present the excision results in a frame shift that terminates protein synthesis thus generating a complete knock-out allele. |
| Phenotypic information | Homozygous:No gross phenotype present. Mice are viable and fertile and pups born at the expected Mendelian ratio.Heterozygous:No gross phenotype present. Mice are viable and fertile and pups born at the expected Mendelian ratio. |
| Breeding history | Bcar1 floxed mice currently maintained on a mixed 129 and C57BL/6J background. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Docking Protein p130Cas Regulates Acinar to Ductal Metaplasia During Pancreatic Adenocarcinoma Development and Pancreatitis.;Costamagna Andrea, Natalini Dora, Camacho Leal Maria Del Pilar, Simoni Matilde, Gozzelino Luca, Cappello Paola, Novelli Francesco, Ambrogio Chiara, Defilippi Paola, Turco Emilia, Giovannetti Elisa, Hirsch Emilio, Cabodi Sara, Martini Miriam, ;2022;Gastroenterology;162;1242-1255.e11; 34922945
- Conditional ablation of p130Cas/BCAR1 adaptor protein impairs epidermal homeostasis by altering cell adhesion and differentiation.;Camacho Leal Maria Del Pilar, Costamagna Andrea, Tassone Beatrice, Saoncella Stefania, Simoni Matilde, Natalini Dora, Dadone Aurora, Sciortino Marianna, Turco Emilia, Defilippi Paola, Calautti Enzo, Cabodi Sara, ;2018;Cell communication and signaling : CCS;16;73; 30390666