Tmprss9 lox/lox
| Status | Available to order |
| EMMA ID | EM:15178 |
| Citation information | RRID:IMSR_EM:15178 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | Tmprss9 lox/lox |
| Alternative name | Tmprss9 lox/lox |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Tmprss9em-lox |
| Gene/Transgene symbol | Tmprss9 |
Information from provider
| Provider | Edith Hummler |
| Provider affiliation | Department of Biomedical Sciences, UNIL |
| Genetic information | Two loxP sites, the recognition sites for the cre-recombinase, were inserted via CRISPR/Cas9 technology, flanking a crucial early exon of Tmprss9. Deletion of exon 3 leads to a frame shift and a premature STOP codon in the following exon. Here, specific CRISPR/Cas9 complexes for the insertion of the loxP-sites were injected directly into mouse oocytes together with a single stranded template for homologous recombination that contains the two loxP-sites, flanking exon 3. For this purpose, no selection cassette is necessary. |
| Phenotypic information | Homozygous:No phenotype.Heterozygous:No phenotype. |
| Breeding history | Particular attention was paid to the design of the sgRNAs through the transgenic service (University of Zurich, Switzerland) in order to obtain a none-to-low off-target score. Once the mice had been obtained in 2019, we have immediately performed back-crossing with C57BL/6N wild-type mice and started the experiments for the recent publication from N=6-7 onwards. Meanwhile, we are at N=10 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | homozygous C57BL/6N males |
Literature references
- Combination of genetic studies and animal modeling proposes TMPRSS9 as a candidate gene for serum K+ variations.;Auberson Muriel, Wang Dongmei, Ehret Elodie, Corre Tanguy, Anand Deepika, Mechakra Asma, Staub Olivier, Bochud Murielle, Hummler Edith, ;2025;Scientific reports;15;25211; 40652049