Sprague Dawley rat GR+/em2
| Status | Available to order |
| EMMA ID | EM:15185 |
| International strain name | Sprague Dawley rat GR+/em2 |
| Alternative name | Sprague Dawley rat GR+/em2 |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Nr3c1 |
| Gene/Transgene symbol | Nr3c1 |
Information from provider
| Provider | Edith Hummler |
| Provider affiliation | Department of Biomedical Sciences, UNIL |
| Genetic information | Deletion at level of the DNA dimerization domain of the rat glucocorticoid receptor (GR) gene. This mutation was described in Ponce de León et al. 2014 as the 11.4, -6 bp deletion and consists of a 7 bp deletion leading to an out-of-frame deletion causing absence of the GR protein product. This mutation leads to a formal GR knockout. The purpose of this line is to assess the function of GR monomers in vivo. |
| Phenotypic information | Homozygous:lethalHeterozygous:adrenal gland hyperplasia and glucocorticoid excess similar to glucocorticoid resistance syndrome. Hypertension under high salt diet. |
| Breeding history | generated in 2013, on going |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous rat Sprague-Dawley , wild-type rat Sprague-Dawley Charles River Laboratories |
| Stage of embryos | 2-cell |
Literature references
- Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.;Ponce de León Verónica, Mérillat Anne-Marie, Tesson Laurent, Anegón Ignacio, Hummler Edith, ;2014;PloS one;9;e88146; 24523878
- Role of glucocorticoid receptor mutations in hypertension and adrenal gland hyperplasia.;Verouti Sophia, Hummler Edith, Vanderriele Paul-Emmanuel, ;2022;Pflugers Archiv : European journal of physiology;474;829-840; 35732960
- Salt-Sensitive Hypertension in GR+/- Rats Is Accompanied with Dysregulation in Adrenal Soluble Epoxide Hydrolase and Polyunsaturated Fatty Acid Pathways.;Vanderriele Paul-Emmanuel, Wang Qing, Mérillat Anne-Marie, Ino Frédérique, Aeschlimann Gilles, Ehret Xavier, Ancin Del Olmo David, Ponce de León Verónica, Scholl Ute I, Winter Denise V, Odermatt Alex, Hummler Edith, Verouti Sophia N, ;2021;International journal of molecular sciences;22;; 34948014