C57BL/6-Tcf21tm(rtTA)/H
| Status | Available to order |
| EMMA ID | EM:15189 |
| International strain name | C57BL/6-Tcf21tm(rtTA)/H |
| Alternative name | TCF21-rtTA |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | TCF21-rtTA |
| Gene/Transgene symbol | Tcf21 |
Information from provider
| Provider | Christopher Switzer |
| Provider affiliation | Queen Mary University of London |
| Genetic information | Reverse Tet-off rtTA protein controlled by Tcf21 promoter (fibroblast-specific). A 9.1 kb region was first subcloned from a positively identified C57BL/6 fosmid clone (WI1-336E22) and used to build the targeting vector using homologous recombination-based techniques. The region is designed so that the long homology arm (LA) extends 6.2 kb upstream of the P2A-rtTA knock-in cassette and the short homology arm (SA) extends 2.3 kb downstream of the neo cassette. The P2A-rtTA cassette was inserted upstream of the TGA stop codon in exon 2 of the mouse Tcf21 gene. The FRT flanked neo selection cassette followed downstream of the 3’-UTR sequence. The targeting vector was confirmed by restriction analysis and sequencing after each modification step. The boundaries of the 2 homology arms were confirmed by sequencing with primers P6 and T73 that read through both sides of the backbone vector into the genomic sequences. The P2A-rtTA knock-in cassette was confirmed by sequencing with primers TCFC SQ1 and SQ2. IV-neo N3 and ineo N2 primers, reading from the selection cassette, confirmed the insertion of the neo cassette. The PTRE3G promoter has been used. |
| Phenotypic information | Homozygous:No observable phenotypic differences.Heterozygous:No observable phenotypic differences. |
| Breeding history | |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |