C57BL/6-Tcf21tm1Cstw/H

Status

Available to order

EMMA IDEM:15189
Citation informationRRID:IMSR_EM:15189 

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International strain nameC57BL/6-Tcf21tm1Cstw/H
Alternative nameTCF21-rtTA
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolTcf21-rtTA
Gene/Transgene symbolTcf21

Information from provider

ProviderChristopher Switzer
Provider affiliationQueen Mary University of London
Genetic informationReverse Tet-off rtTA protein controlled by Tcf21 promoter (fibroblast-specific). A 9.1 kb region was first subcloned from a positively identified C57BL/6 fosmid clone (WI1-336E22) and used to build the targeting vector using homologous recombination-based techniques. The region is designed so that the long homology arm (LA) extends 6.2 kb upstream of the P2A-rtTA knock-in cassette and the short homology arm (SA) extends 2.3 kb downstream of the neo cassette. The P2A-rtTA cassette was inserted upstream of the TGA stop codon in exon 2 of the mouse Tcf21 gene. The FRT flanked neo selection cassette followed downstream of the 3’-UTR sequence. The targeting vector was confirmed by restriction analysis and sequencing after each modification step. The boundaries of the 2 homology arms were confirmed by sequencing with primers P6 and T73 that read through both sides of the backbone vector into the genomic sequences. The P2A-rtTA knock-in cassette was confirmed by sequencing with primers TCFC SQ1 and SQ2. IV-neo N3 and ineo N2 primers, reading from the selection cassette, confirmed the insertion of the neo cassette. The PTRE3G promoter has been used.
Phenotypic informationHomozygous:
No observable phenotypic differences.

Heterozygous:
No observable phenotypic differences.
Breeding history
ReferencesNone available
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (gene matching)
  • abnormal spleen morphology / IMPC
  • abnormal placenta morphology / IMPC
  • persistence of hyaloid vascular system / IMPC
  • embryonic growth retardation / IMPC
  • small spleen / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • abnormal eye morphology / IMPC
  • abnormal ovary morphology / IMPC
  • abnormal kidney morphology / IMPC
  • abnormal testis morphology / IMPC
  • edema / IMPC
  • abnormal blood vessel morphology / IMPC
  • microphthalmia / IMPC
  • increased circulating calcium level / IMPC
  • enlarged kidney / IMPC
  • enlarged testis / IMPC
  • anophthalmia / IMPC
  • abnormal placenta vasculature / IMPC

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
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Material Transfer Agreement (MTA)
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