B6;CBA-Tg(H4C16-mCherry)/Cnbc
| Status | Available to order |
| EMMA ID | EM:15239 |
| Citation information | RRID:IMSR_EM:15239 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;CBA-Tg(H4C16-mCherry)/Cnbc |
| Alternative name | B6.H4-mCherry |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(H4C16-mCherry) |
| Gene/Transgene symbol | Tg(H4C16-mCherry) |
Information from provider
| Provider | Lluís Espinosa |
| Provider affiliation | Cancer, IMIM (Institut de Recerca Hospital del Mar) |
| Additional owner | PBRPB CAG-mCherry-IRES-PURO plasmid from Addgene (#106333) was used for the generation of this mutant. |
| Genetic information | A Homo sapiens H4 histone 16 (H4C16) mRNA (NCBI Reference Sequence: NM_175054.2) was fused to an mCherry protein-coding sequence. This construct was cloned into piggy bac transposon system. The line was generated by pronuclear injection of B6CBA zygotes with 30 ng/μl hyPBase mRNA+ 30 ng/μl PB_CAG_Cherry-histoneH4 plasmid (BRPB CAG-mCherry-IRES-PURO Addgene #106333) and transfer into pseudo-pregnant 0.5 dpc CD1 females. |
| Phenotypic information | Homozygous:Unknown.Heterozygous:No phenotypic effects in heterozygous mice. |
| Breeding history | A male H4-mCherry from the first generation was crossed with C57BL/6 females and transgene positive male offspring were subsequently mated to C57BL/6 females. This mating scheme was repeated for 17 generations. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
Literature references
- Dynamic chromatin association of IκBα is regulated by acetylation and cleavage of histone H4.;Marruecos Laura, Bertran Joan, Álvarez-Villanueva Daniel, Mulero María Carmen, Guillén Yolanda, Palma Luis G, Floor Martin, Vert Anna, Arce-Gallego Sara, Pecharroman Irene, Batlle Laura, Villà-Freixa Jordi, Ghosh Gourisankar, Bigas Anna, Espinosa Lluís, ;2021;EMBO reports;22;e52649; 34224210