BALB/c-Cd1d2em1Kblg/Orl
| Status | Available to order |
| EMMA ID | EM:15249 |
| Citation information | RRID:IMSR_EM:15249 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | BALB/c-Cd1d2em1Kblg/Orl |
| Alternative name | BALB/C CD1d2 KO |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Cd1d2em1Kblg |
| Gene/Transgene symbol | Cd1d2 |
Information from provider
| Provider | Kamel Benlagha |
| Provider affiliation | Immunology, IRSL-Inserm U1160 |
| Genetic information | Mutation in exon 1 of the Cd1d2 gene: a T at position 23 was deleted in founders inducing a downstream STOP codon. Thus, from the start codon ATG to the new STOP, a 38-aa protein was produced. CRISPR technology details: Cas9 mRNA and sgRNAs targeting exon1 were mixed and electroporated (NEPA21) into the pronuclei of the one-cell embryos. SgRNA2 5′-CCTACCGTGCCTGTTGCTGT-3′ and SgRNA4 5′-CAGCAACAGGCAC GGTAGGT-3′. |
| Phenotypic information | Homozygous:Mice deficient in innate natural killer T (NKT) cell subsets.Heterozygous:Altered distribution of NKT cell subsets. |
| Breeding history | Mutation generated by CRISPR/Cas9 technology on BALB/c background. Maintained by breeding of homozygous mice. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | homozygous BALB/c males |
Literature references
- Intrinsic factors and CD1d1 but not CD1d2 expression levels control invariant natural killer T cell subset differentiation.;Amable Ludivine, Ferreira Martins Luis Antonio, Pierre Remi, Do Cruseiro Marcio, Chabab Ghita, Sergé Arnauld, Kergaravat Camille, Delord Marc, Viret Christophe, Jaubert Jean, Liu Chaohong, Karray Saoussen, Marie Julien C, Irla Magali, Georgiev Hristo, Clave Emmanuel, Toubert Antoine, Lucas Bruno, Klibi Jihene, Benlagha Kamel, ;2023;Nature communications;14;7922; 38040679