B6J.Cg-Atpif1tm1-(EUCOMM)Wtsi/Cnbc
| Status | Available to order |
| EMMA ID | EM:15298 |
| International strain name | B6J.Cg-Atpif1tm1-(EUCOMM)Wtsi/Cnbc |
| Alternative name | Atp5if1-global KO also named Global IF1 knockout |
| Strain type | Targeted Mutant Strains |
| Allele/Transgene symbol | loss of exon 3 in Atp5if1 gene |
| Gene/Transgene symbol | Atp5if1 |
Information from provider
| Provider | José M Cuezva |
| Provider affiliation | Centro de Biología Molecular, Universidad Autónoma de Madrid |
| Genetic information | We used the Atp5if1-floxed mouse line generated and described in our PLoS Biol 2021 publication (doi.org/10.1371/journal.pbio.3001252) and the B6.Cg-Tg(Camk2a-cre)T29-1Stl/J mouse strain. The latter expresses cre recombinase in neurons and in the germline. Germline recombination of the floxed Atp5if1 (IF1) allele was assessed by PCR in different tissues and its inheritance by breeding with wild-type C57BL/6J mice verified in tissues that naturally express IF1, which showed no expression of the protein (see Commun Biol 2023, 6:836, doi.org/10.1038/s42003-023-05214-1). |
| Phenotypic information | Homozygous:None except when stressedHeterozygous:Not studied. |
| Breeding history | 6-7 generations in C57BL/6J. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6J |