C57BL/6N-Mcpt8^{tm2.1(cre/ERT2)Ics}/Ics

Status	Available to order
EMMA ID	EM:15551
Citation information	RRID:IMSR_EM:15551 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-Mcpt8^{tm2.1(cre/ERT2)Ics}/Ics
Alternative name	Mcpt8tm2.1(RFP657/cre/ERT2)Ics
Strain type	Targeted Mutant Strains : Knock-in
Allele/Transgene symbol	Mcpt8^{tm2.1(cre/ERT2)Ics}
Gene/Transgene symbol	Mcpt8

Information from provider

Provider	ICS, Institut Clinique de la Souris
Provider affiliation	ICS, Institut Clinique de la Souris
Genetic information	This line was obtained by modification of S3 C57BL/6N embryonic stem cells. A cassette was inserted in exon 1 of Mcpt8, by homologous recombination in ES cells. An intron was added in exon 1 to stabilize the transgenic cDNA which contains a mutated TagRFP657 far-red monomeric fluorescent protein sequence, an LF2A (2A self-cleaving peptide) sequence which can induce ribosomal skipping during translation, a dual cre-F3-ERT2-F3 cassette which allows expression of creERT2 or cre (if crossed with an flp deleter), and a selection cassette. The pPGK-Neo selection cassette flanked by rox attachment sites was removed by breeding with the dre recombinase deleter line C57BL/6N-Gt(ROSA)26Sor-tm4.1(CAG-dre)Ics (EMMA strain ID EM:15557). TagRFP657 is not detected due to the mutation in its cDNA. A detailed model generation technical report is enclosed. For detailed information on the genetic description of this strain, please have a look at this report.
Phenotypic information	Homozygous: NA Heterozygous: NA
Breeding history	C57BL/6N inbred (4 generations).
References	None available
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Mcpt8tm2.1(cre/ERT2)Ics/Ics