C57BL/6N-Mcpt8^{tm1.1(cre)Ics}/Ics

Status	Available to order
EMMA ID	EM:15553
Citation information	RRID:IMSR_EM:15553 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-Mcpt8^{tm1.1(cre)Ics}/Ics
Alternative name	Mcpt8tm1.1(Cre/TdTomato)Ics
Strain type	Targeted Mutant Strains : Knock-in
Allele/Transgene symbol	Mcpt8^{tm1.1(cre)Ics}
Gene/Transgene symbol	Mcpt8

Information from provider

Provider	Nicolas Charles
Provider affiliation	Centre de Recherche sur l, Université Paris Cité
Genetic information	This line was obtained by modification of S3 C57BL/6N embryonic stem cells. A Cre-T2A-TdTomato cassette was inserted after the STOP codon of Mcpt8, by homologous recombination in ES cells. The pPGK-Neo selection cassette flanked by FRT sites was removed by breeding chimeras with a C57BL/6N flp recombinase deleter line (EMMA strain ID EM:05490). This line has been published, with description of cre recombinase and Td-Tomato specific expression in basophils. A detailed technical report of model generation is also enclosed. For detailed information on the genetic description of this strain, please see this report.
Phenotypic information	Homozygous: NA Heterozygous: NA
Breeding history	Inbred to C57BL/6N for 2 generations.
References	CT-M8 Mice: A New Mouse Model Demonstrates That Basophils Have a Nonredundant Role in Lupus-Like Disease Development.;Tchen John, Simon Quentin, Chapart Léa, Pellefigues Christophe, Karasuyama Hajime, Miyake Kensuke, Blank Ulrich, Benhamou Marc, Daugas Eric, Charles Nicolas, ;2022;Frontiers in immunology;13;900532; 35844602
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

Literature references

CT-M8 Mice: A New Mouse Model Demonstrates That Basophils Have a Nonredundant Role in Lupus-Like Disease Development.;Tchen John, Simon Quentin, Chapart Léa, Pellefigues Christophe, Karasuyama Hajime, Miyake Kensuke, Blank Ulrich, Benhamou Marc, Daugas Eric, Charles Nicolas, ;2022;Frontiers in immunology;13;900532; 35844602

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6N-Mcpt8tm1.1(cre)Ics/Ics