BALB/cN-Prg2tm2(RFP657/cre/ERT2)Ics/Ics
| Status | Available to order |
| EMMA ID | EM:15555 |
| International strain name | BALB/cN-Prg2tm2(RFP657/cre/ERT2)Ics/Ics |
| Alternative name | Prg2tm2(RFP657/cre/ERT2)Ics |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Prg2tm2(RFP657/cre/ERT2)Ics |
| Gene/Transgene symbol | Prg2 |
Information from provider
| Provider | ICS, Institut Clinique de la Souris |
| Provider affiliation | ICS, Institut Clinique de la Souris |
| Genetic information | This line was obtained by modification of BA3 BALB/cN embryonic stem cells. A 5.6 kb trap cassette was inserted by homologous recombination in ES cells in the intronic region of Prg2 located between exon 2 and exon 3. This cassette contains intron 1 and partial exon sequence of En2 to act as splice donor site, a LF2A (2A self-cleaving peptide) sequence that can induce ribosomal skipping during translation, a mutated TagRFP657 far-red monomeric fluorescent protein sequence, a T2A sequence and a dual cre-F3-ERT2-F3 cassette that allow expression of creERT2 or cre (if crossed with a flp deleter) and a selection cassette. The pPGK-Neo selection cassette flanked by rox attachment sites was not removed. FACS analysis showed efficient and specific cre expression in spleen eosinophils. TagRFP657 is not detected due to a mutation in its cDNA. For detailed information on the genetic description of this strain, please have a look at this report. |
| Phenotypic information | Homozygous:NAHeterozygous:NA |
| Breeding history | BALB/cN inbred (4 generations). |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France |