C57BL/6N-Tg(Nr2f2-cre/ERT2)40Ics/Ics

Status	Available to order
EMMA ID	EM:15558
International strain name	C57BL/6N-Tg(Nr2f2-cre/ERT2)40Ics/Ics
Alternative name	Tg(Nr2f2-cre/ERT2)40.ICS
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Nr2f2-cre/ERT2)40.ICS
Gene/Transgene symbol	Tg(Nr2f2-cre/ERT2)40.ICS

Information from provider

Provider	ICS, Institut Clinique de la Souris
Provider affiliation	ICS, Institut Clinique de la Souris
Genetic information	The line was obtained by microinjection of a circular murine BAC transgene (RP23-109L9), previously modified by recombineering at the ATG of the Nrf2f2 gene of a Cre-F3-ERT2-F3 dual cassette followed by a roxed PGK-Tn5-Kana/NeoR cassette. The genetic background of the fertilized oocytes was C57BL/6N. The line was then maintained in a C57BL/6N genetic background. The roxed PGK-Tn-Kana/NeoR cassette was not removed. The subline 40 was analyzed and cryopreserved. The number of transgenic copies integrated into the genome was estimated to be one per ddPCR. The corresponding integration site was not searched. The expression of cre mRNA was observed at low level in heart, ovary and uterus (respectively 5, 1 and 2% of Nr2f2 endogenous expression). For detailed information on the genetic description of this strain, please have a look at this report.
Phenotypic information	Homozygous: NA Heterozygous: NA
Breeding history	C57BL/6N inbred (3 generations).
References	None available
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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