C57BL/6N-Tg(Nr2f2-cre)40.1Ics/Ics

Status	Available to order
EMMA ID	EM:15559
Citation information	RRID:IMSR_EM:15559 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-Tg(Nr2f2-cre)40.1Ics/Ics
Alternative name	Tg(Nr2f2-cre)40.ICS
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Nr2f2-cre)40.ICS
Gene/Transgene symbol	Tg(Nr2f2-cre)40.1Ics

Information from provider

Provider	ICS, Institut Clinique de la Souris
Provider affiliation	ICS, Institut Clinique de la Souris
Genetic information	This line was obtained by crossing Tg(Nr2f2-cre/ERT2)40.ICS (EMMA strain ID 15558) animals with the C57BL/6NTac-Gt(ROSA)26Sor-tm2(CAG-flpo,-EYFP)Ics (EMMA strain ID EM:05490) ubiquitous flp deleter resulting in a cre-F3 sequence located at the ATG of the Nrf2f2 gene followed by a roxed PGK-Tn5-Kana/NeoR cassette. The pure C57BL/6N background was maintained for all breeding steps. Efficiency of deletion was confirmed by crossing this line with the cre recombinase-inducible fluorescent reporter Gt(ROSA)26Sor-tm4(ACTB-tdTomato,-EGFP)Luo. EGFP labeling was observed in a structure next to the ovary which could be the oviduct/uterus transition, no labeling in the oviduct was observed. In the heart, EGFP labelling is observed in some cardiomyocytes. In uterus, EGFP labelling is observed in smooth muscle cells. In bladder, EGFP labelling is observed in few smooth muscle cells. For detailed information on the genetic description of this strain, please have a look at this report.
Phenotypic information	Homozygous: NA Heterozygous: NA
Breeding history	Inbred to C57BL/6N for 3 generations.
References	None available
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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