C57BL/6N-Is(7TOMM40,APOE_i4,APOC1)3.2Ics/Ics

Status	Available to order
EMMA ID	EM:15564
Citation information	RRID:IMSR_EM:15564 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6N-Is(7TOMM40,APOE_i4,APOC1)3.2Ics/Ics
Alternative name	ApoE tm1(TOMM40; APOE4; APOC1)Ics
Strain type	Endonuclease-mediated
Allele/Transgene symbol	Is(7TOMM40,APOE_i4,APOC1)3.2Ics
Gene/Transgene symbol	Is(7TOMM40,APOE_i4,APOC1)3.2Ics

Information from provider

Provider	Marie-Christine Birling
Provider affiliation	Genetic Engineering and Model Validation, PHENOMIN-ICS
Genetic information	This line was obtained by modification of S3 in house-derived C57BL/6N embryonic stem cells. The murine Tomm40, ApoE and Apoc1 28.2 kb region was replaced by a 34.2 kb human genomic sequence that contains TOMM40, APOE4 and APOC1. The floxed HygroR cassette and the flipped NeoR cassette were removed by 2 successive breeding steps.
Phenotypic information	Homozygous: Not done. Heterozygous: No obvious phenotype observed on young animals.
Breeding history	The line was generated on a C57BL/6NCrl genetic background and remained on the same genetic background. The cre (EMMA strain ID EM:05488) and the flp (EMMA strain ID EM:05490) deleter lines were also generated on the same genetic background.
References	None available
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

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