B6J(B10)-Ptprcem1Jmar Tg(CD2-TcraF5,CD2-TcrbF5)1Kio/Orl

Status

Available to order

EMMA IDEM:15572
Citation informationRRID:IMSR_EM:15572 

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International strain nameB6J(B10)-Ptprcem1Jmar Tg(CD2-TcraF5,CD2-TcrbF5)1Kio/Orl
Alternative nameF5 x CD45.1 or B6-Ptprcem(K302E)Jmar/J -Tg(CD2-TcraF5,CD2-TcrbF5)1Kio/Jmar
Strain typeEndonuclease-mediated
Allele/Transgene symbolPtprcem1Jmar, Tg(CD2-TcraF5,CD2-TcrbF5)1Kio
Gene/Transgene symbolPtprc, Tg(CD2-TcraF5,CD2-TcrbF5)1Kio

Information from provider

ProviderJacqueline Marvel
Provider affiliationCIRI INSERM U1111
Genetic informationThis strain is transgenic for the F5 TCR, that recognizes the NP68 peptide from influenza A virus (ASNENMDAM) in the context of H2-Db. It also contains a mutation in the amino acid n. 302 of the Ptprc gene leading to an amino acid change (K to E). This mutation was generated by CRISPR/Cas9 genome editing and these mice express the Ptprca (CD45.1) rather than the Ptprcb (CD45.2) isoform, normally expressed by C57BL/6J mice. CRISPR technology details: the Cas9 protein from Streptococcus pyogenes with NLS was purchased from PNA BIo (Cat. n. CP02). The F5 transgenic mice have been crossed with the B6J-Ptprcem(K302E)Jmar mice in order to express the PTPRCa (CD45.1) isoform of the PTPRC (CD45) protein. The CD8 T cells from the F5 x CD45.1 (Tg(CD2-TcraF5,CD2-TcrbF5)1Kio/Jmar x B6J-Ptprcem(K302E)Jmar) mice can thus be transferred in a C57BL/6J host, and easily followed in the host by flow cytometry, using an anti-CD45.1 antibody. This mouse strain is a valuable tool for studying CD8 T cell response, as all the CD8 T cells share the same specificity for NP68 peptide.
Phenotypic informationHomozygous:
Hematopoietic cells of the mouse express the CD45.1 epitope instead on the CD45.2 epitope. All the CD8 T cells express the same F5 TCR.

Heterozygous:
Hematopoietic cells of the mouse express both the CD45.1 epitope and the CD45.2 epitope. Half of them will express the F5 TCR.
Breeding historyThis strain is a result of a cross between: 1) B6J-Ptprc302K/E/Jmar: a C57BL/6J strain that contains a single amino acid mutation in the gene encoding the PTPRC (or CD45) protein, allowing the expression of the CD45.1 isoform, instead of the CD45.2 isoform. This strain is also available from INFRAFRONTIER (EMMA strain EM:15082). 2) B6J(B10)-Tg(CD2-TcraF5,CD2-TcrbF5)1Kio/Jmar: C57BL/10-Tg(Cd2-TcraF5,CD2-TcrbF5)1Kio/AnuApb mice, provided by Prof. D. Kioussis (National Institute of Medical Research, London, U.K.) and backcrossed on C57BL/6J background (published in: Jubin V et al.; T inflammatory memory CD8 T cells participate to antiviral response and generate secondary memory cells with an advantage in XCL1 production; Immunol Res. 2012 Jun;52(3):284-93. doi:10.1007/s12026-012-8340-4. PMID:22528127).
References
  • Generation of a C57BL/6J mouse strain expressing the CD45.1 epitope to improve hematopoietic stem cell engraftment and adoptive cell transfer experiments.;Laubreton Daphné, Djebali Sophia, Angleraux Céline, Chain Benny, Dubois Maxence, Henry Farida, Leverrier Yann, Teixeira Marie, Markossian Suzy, Marvel Jacqueline, ;2023;Lab animal;52;324-331; 38017180
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredyes
Immunocompromisedno

Information from EMMA

Archiving centreCNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France
Animals used for archivingheterozygous C57BL/6J males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (gene matching)
  • abnormal long bone metaphysis morphology / MGI
  • abnormal leukocyte cell number / MGI
  • increased neutrophil cell number / MGI
  • decreased leukocyte cell number / MGI
  • extramedullary hematopoiesis / MGI
  • abnormal erythropoiesis / MGI
  • enlarged spleen / MGI
  • spleen hyperplasia / MGI
  • enlarged lymph nodes / MGI
  • decreased thymocyte number / MGI
  • abnormal immune system cell morphology / MGI
  • abnormal myelination / MGI
  • abnormal oligodendrocyte morphology / MGI
  • abnormal osteoclast physiology / MGI
  • abnormal digestion / MGI
  • abnormal immune system physiology / MGI
  • abnormal humoral immune response / MGI
  • thymus hypoplasia / MGI
  • arrested T cell differentiation / MGI
  • abnormal T cell activation / MGI
  • liver inflammation / MGI
  • lung inflammation / MGI
  • premature death / MGI
  • abnormal T cell differentiation / MGI
  • no abnormal phenotype detected / MGI
  • abnormal bone marrow cell morphology/development / MGI
  • abnormal lymphopoiesis / MGI
  • abnormal double-positive T cell morphology / MGI
  • increased susceptibility to viral infection / MGI
  • abnormal T cell physiology / MGI
  • abnormal B cell number / MGI
  • increased IgA level / MGI
  • abnormal immune system organ morphology / MGI
  • glomerulonephritis / MGI
  • increased urine protein level / MGI
  • abnormal cytokine secretion / MGI
  • no phenotypic analysis / MGI
  • kidney failure / MGI
  • oliguria / MGI
  • abnormal thymocyte activation / MGI
  • abnormal T cell subpopulation ratio / MGI
  • increased anti-double stranded DNA antibody level / MGI
  • abnormal T cell selection / MGI
  • abnormal positive T cell selection / MGI
  • increased B-1 B cell number / MGI
  • abnormal osteoclast morphology / MGI
  • increased B cell number / MGI
  • increased T cell number / MGI
  • decreased B cell number / MGI
  • decreased T cell number / MGI
  • abnormal response to infection / MGI
  • diarrhea / MGI
  • decreased cytotoxic T cell cytolysis / MGI
  • increased double-negative T cell number / MGI
  • decreased double-positive T cell number / MGI
  • decreased B cell proliferation / MGI
  • decreased T cell proliferation / MGI
  • increased susceptibility to autoimmune disorder / MGI
  • immune system phenotype / MGI
  • hematopoietic system phenotype / MGI
  • decreased susceptibility to type I hypersensitivity reaction / MGI
  • CNS inflammation / MGI
  • increased T cell apoptosis / MGI
  • abnormal T cell morphology / MGI
  • increased NK cell number / MGI
  • abnormal memory T cell number / MGI
  • increased memory T cell number / MGI
  • decreased memory T cell number / MGI
  • absent T cells / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • abnormal CD4-positive T cell differentiation / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • abnormal CD8-positive, alpha-beta T cell differentiation / MGI
  • increased single-positive T cell number / MGI
  • decreased single-positive T cell number / MGI
  • increased plasma cell number / MGI
  • lymph node hyperplasia / MGI
  • abnormal granulocyte differentiation / MGI
  • increased dendritic cell number / MGI
  • decreased B-1a cell number / MGI
  • increased B-1b cell number / MGI
  • decreased B-1b cell number / MGI
  • decreased follicular B cell number / MGI
  • increased transitional stage B cell number / MGI
  • abnormal follicular B cell physiology / MGI
  • decreased B-2 B cell number / MGI
  • decreased mature B cell number / MGI
  • abnormal B cell activation / MGI
  • abnormal leukocyte morphology / MGI
  • decreased gamma-delta intraepithelial T cell number / MGI
  • abnormal CD4-positive, alpha-beta intraepithelial T cell morphology / MGI
  • increased spleen red pulp amount / MGI
  • increased spleen white pulp amount / MGI
  • increased IgG2a level / MGI
  • increased plasmacytoid dendritic cell number / MGI
  • abnormal chemokine secretion / MGI
  • decreased memory B cell number / MGI
  • decreased mast cell degranulation / MGI
  • abnormal intraepithelial T cell morphology / MGI
  • abnormal intraepithelial T cell number / MGI
  • decreased oligodendrocyte number / MGI
  • increased DN3 thymocyte number / MGI
  • decreased DN4 thymocyte number / MGI
  • abnormal NK cell physiology / MGI
  • mortality/aging / MGI
  • decreased CD4-positive, alpha-beta memory T cell number / MGI
  • decreased CD8-positive, alpha-beta memory T cell number / MGI
  • lethality at weaning, incomplete penetrance / MGI
  • increased alpha-beta T cell number / MGI
  • decreased CD8-positive, naive alpha-beta T cell number / MGI

Literature references

  • Generation of a C57BL/6J mouse strain expressing the CD45.1 epitope to improve hematopoietic stem cell engraftment and adoptive cell transfer experiments.;Laubreton Daphné, Djebali Sophia, Angleraux Céline, Chain Benny, Dubois Maxence, Henry Farida, Leverrier Yann, Teixeira Marie, Markossian Suzy, Marvel Jacqueline, ;2023;Lab animal;52;324-331; 38017180

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

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Practical information

Genotyping protocol

Example health report
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Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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