Irx1-(creERT2-2a-Venus)Schei/Orl
| Status | Under development - register interest |
| EMMA ID | EM:15635 |
| Citation information | RRID:IMSR_EM:15635 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | Irx1-(creERT2-2a-Venus)Schei/Orl |
| Alternative name | Tg(RP24-370F6)IRX1(creERT2-2a-Venus)Schei |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Irx1(CreERT2-T2A-Venus) |
| Gene/Transgene symbol | Irx1(CreERT2-T2A-Venus) |
Information from provider
| Provider | Peter Scheiffele |
| Provider affiliation | Biozentrum University of Bas |
| Genetic information | BAC transgenic mice where a creERT2-T2A-Venus open reading frame was positioned at the translational start site in the Irx1 exon 1. Specifically, BAC transgenic mice expressing a CreERT2-2A-Venus fusion protein were generated using a two-plasmid approach for BAC modification (PMID:20885380). BAC RP24-370F6 was obtained from the BACPAC resource at Children’s Hospital Oakland. This BAC spans all exons of the mouse Irx1 gene and 68,500 bps upstream and ca. 90,000 bps downstream sequence. A 283 bp region immediately upstream of the ATG start site was chosen as A-homology arm, followed by a CreERT2-T2A-Venus open reading frame positioned at the translational start site in Irx1 exon 1. This open reading frame is followed by a bovine growth hormone poly-adenylation sequence. BAC transgenic mice were generated by pronuclear injection and insertion-positive founders were identified by PCR. |
| Phenotypic information | Homozygous:Tamoxifen induction of cre recombinase activity results in selective cre-mediated recombination in unipolar brush cells in the cerebellum, interneurons in layer 1, and a population of neurons in the hippocampus. At high tamoxifen doses recombination is observed in some Bergmann glia cells. Relative abundances of cell types are also dependent on developmental stage (postnatal age) of tamoxifen application. Heterozygous:Tamoxifen induction of cre recombinase activity results in selective cre-mediated recombination in unipolar brush cells in the cerebellum, interneurons in layer 1, and a population of neurons in the hippocampus. |
| Breeding history | Backcrossed to C57BL/6JRj for more than 6 generations. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |