C57BL/6-Slc44a1^em1Cnbc/Cncb

Status	Available to order
EMMA ID	EM:15672
Citation information	RRID:IMSR_EM:15672 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6-Slc44a1^em1Cnbc/Cncb
Alternative name	Slc44a1( KO exon 2)
Strain type	Endonuclease-mediated
Allele/Transgene symbol	Slc44a1^em1Cnbc
Gene/Transgene symbol	Slc44a1

Information from provider

Provider	Ekaitz Errasti Murugarren
Provider affiliation	Department of Physiological Sciences, , School of Medicine, University of Barcelona
Genetic information	The Slc44a1 mouse line was created using CRISPR/Cas9 technology, p.Arg15Glndelfsx34: deletion of 1 bp in exon 2, generating a truncated protein from amino acid 15 (arginine). These mice express the Arg15Glndelfsx34 protein in the Slc44a1 gene. CRISPR technology details: The mouse model was generated using Cas9 protein and guide RNA (crRNA+tracrRNA) and therefore the coding sequence for Cas9 has not been integrated in the mouse genome.
Phenotypic information	Homozygous: Unknown Heterozygous: Unknown
Breeding history	The mouse line was generated in C57BL/6 genetic background. Less than 10 generations.
References	None available
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

IMPC phenotypes (gene matching)

abnormal coat appearance / IMPC
decreased total retina thickness / IMPC
abnormal bone structure / IMPC
irregularly shaped pupil / IMPC
decreased prepulse inhibition / IMPC
increased heart rate / IMPC
increased bone mineral content / IMPC
abnormal retina blood vessel morphology / IMPC
abnormal retina outer nuclear layer morphology / IMPC
abnormal eye anterior chamber depth / IMPC
hyperactivity / IMPC
increased vertical activity / IMPC
abnormal eye posterior chamber depth / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

The information provided on this page is, to the best of EMMA’s knowledge, based on data supplied by the original provider. End users are responsible for reviewing these details and for validating the strain and its suitability for their experimental use.
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C57BL/6-Slc44a1em1Cnbc/Cncb