B6(Cg)-Xpftm(avi)Ggar Tg(Hnrnpa2b1/Cbx1-birA)1Jstr/Flmg
| Status | Available to order |
| EMMA ID | EM:15689 |
| Citation information | RRID:IMSR_EM:15689 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6(Cg)-Xpftm(avi)Ggar Tg(Hnrnpa2b1/Cbx1-birA)1Jstr/Flmg |
| Alternative name | B6(Cg)-XPFavi Tg(Hnrnpa2b1/Cbx1-birA)1Ggar/FORTH or bXPF or avXpf;BirA |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Xpftm(avi)Ggar, Tg(Hnrnpa2b1/Cbx1-birA)1Jstr |
| Gene/Transgene symbol | Xpf, Tg(Hnrnpa2b1/Cbx1-birA)1Jstr |
Information from provider
| Provider | George Garinis |
| Provider affiliation | IMBB, ITE FORTH |
| Genetic information | Knock-in mice (avXpf) expressing the NER structure-specific endonuclease XPF fused carboxy terminally, before the stop codon of the last exon 11, with a 15-amino-acid tandem affinity purification (TAP) tag biotinylatable Avi sequence and a 3x Flag tag separated by a tobacco etch virus (TEV) protease cleavage site. The mouse strain is also transgenic for the BirA bacterial biotin ligase under the control of the hnRNPA2 promoter (see description under EMMA strain ID EM:09008). This mouse strain (bXPF or avXpf;BirA) expresses in vivo biotinylated XPF protein in tissues where the Xpf gene is normally active. |
| Phenotypic information | Homozygous:bXPF animals are born at the expected Mendelian frequency, grow normally and show no developmental defects or other pathological features. Homozygous: no overt phenotype.Heterozygous:Heterozygous: no overt phenotype. |
| Breeding history | TAP-tag-fused heterozygous males (avXpf+/-) were backcrossed and maintained in a C57BL/6J background. Homozygous avXpf+/+ knock-in mice were then crossed with mice ubiquitously expressing the HA-tagged bacterial BirA biotin ligase transgene to generate the biotin-tagged XPF (bXPF) mice. This mouse strain has been continuously bred for the last 12 years. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | homozygous C57BL/6 males, wild-type C57BL/6J females |
| Stage of embryos | Morula |
Literature references
- ERCC1-XPF cooperates with CTCF and cohesin to facilitate the developmental silencing of imprinted genes.;Chatzinikolaou Georgia, Apostolou Zivkos, Aid-Pavlidis Tamara, Ioannidou Anna, Karakasilioti Ismene, Papadopoulos Giorgio L, Aivaliotis Michalis, Tsekrekou Maria, Strouboulis John, Kosteas Theodore, Garinis George A, ;2017;Nature cell biology;19;421-432; 28368372