IMPC phenotypes (gene matching)
C57BL/6-Rbm20em1.1Schei/Orl
| Status | Available to order |
| EMMA ID | EM:15702 |
| Citation information | RRID:IMSR_EM:15702 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Rbm20em1.1Schei/Orl |
| Alternative name | B6-Rbm20 tm1.1Schei |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Rbm20em1.1Schei |
| Gene/Transgene symbol | Rbm20 |
Information from provider
| Provider | Pawel Pelczar |
| Provider affiliation | Center for Transgenic Models, University of Basel |
| Genetic information | The knock-in line was generated using CRISPR/Cas9 system by homology-directed repair with a single stranded DNA megamer. The gRNAs targeted the last intron of Rbm20 and single-stranded DNA was introduced together with mRNA encoding for CRISPR/Cas9 nuclease into C57BL/6 zygotes. The surviving embryos were transferred into recipient females. The inserted DNA sequences encoded a conditional by inversion (COIN) module, containing a 3'-splice site which allows the expression of the last exon of the gene fused to a histidin-biotin-histidin-tripleHA tag. The COIN allele is inserted in an orientation opposite to the gene’s direction of transcription. Activation by cre recombinase inverts the COIN module, resulting in splicing of the pre-mRNA to the tagged copy of the exon. As we experienced extremely inefficient conditional inversion when the original knock-in was crossed with cre-driver lines, embryos from the initial COIN allele line were electroporated with cre mRNA. The deposited allele is the result of cre-mediated inversion. Thus, the mice exhibit constitutive expression of the histidin-biotin-histidin-tripleHA tagged RBM20 protein in all cells of the body. |
| Phenotypic information | Homozygous:The line results in the histidin-biotin-histidin-tripleHA tagging of the RBM20 protein. Mice are viable and fertile and express wild-type levels of RBM20 protein. No constraints were identified upon breeding this line for several generations.Heterozygous:As for homozygous. |
| Breeding history | More than 10 generations of backcrossing with C57BL/6J background. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J males |