hGITR KI
| Status | Available to order |
| EMMA ID | EM:15708 |
| Citation information | RRID:IMSR_EM:15708 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | hGITR KI |
| Alternative name | hGITR KI |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Unknown at present |
| Gene/Transgene symbol | Unknown at present |
Information from provider
| Provider | Mark Cragg |
| Provider affiliation | Cancer Sciences, University of Southampton |
| Genetic information | Gene encoding a protein expressing the human GITR extracellular domain with mouse intracellular domain knocked into the mouse GITR genomic locus. The coding sequences of exon 1 plus some immediate intronic sequences were replaced with a KI cassette, thus the expression of the KI cassette will be under control of the mouse Tnfrsf18 gene regulatory element. To engineer the targeting vector, homology arms were generated by PCR using BAC clone RP23-139J18 from the C57BL/6J library as template. In the targeting vector, the Neo cassette was flanked by LoxP sites. |
| Phenotypic information | Homozygous:No deleterious changes. They express a receptor with human GITR extracellular domain, that can be bound by anti-human GITR monoclonal antibody.Heterozygous:Heterozygous mice express half as much hGITR receptor as the homozygous mice. It also deletes one mouse allele and so expresses 50% of the mouse GITR receptor. |
| Breeding history | Mice were generated in C57BL/6 background (ES cells). |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |