hNGFwt
| Status | Under development - register interest |
| EMMA ID | EM:15774 |
| Citation information | RRID:IMSR_EM:15774 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | hNGFwt |
| Alternative name | hNGFwt |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | NgfhNGFwt |
| Gene/Transgene symbol | Ngf |
Information from provider
| Provider | Simona Capsoni |
| Provider affiliation | University of Ferrara |
| Genetic information | We generated mutant mice harboring the human NGF wild-type gene at the mouse Ngf locus (this strain: hNGFwt, EMMA strain EM:15574) to control for the hNGFR100W/wt strain (EMMA strain EM:15575) which is a model for Hereditary Sensory and Autonomic Neuropathy type V. We cannot guarantee the genotype and the recipient will have to take the responsibility to carry out all the necessary genotypic checks (including sequencing of the PCR products to distinguish wild-type from mutant animals). |
| Phenotypic information | Homozygous:Homozygous NGFh/h mice thrive to adulthhod and do not show an alterated pheenotypeHeterozygous:Homozygous NGFh/m mice thrive to adulthood and do not show an alterated phenotype |
| Breeding history | pCMV6-XL5-human NGF was generated using site-specific mutagenesis PCR. The targeting constructs were generated using classical cloning technologies. Briefly, a BAC (bacterial artificial chromosome; clone RP24-160F12) containing the entire regions of interest flanking the NGF sequence, was used to generate intermediate plasmids by cloning in pBluescript SK(-) the 5 homology arm (from 89489 to 94076, restriction site MfeI) and 3 homology arm (from 94803 to 99710, restriction site HindIII). The human NGF coding sequence was cloned in the pKO2.1 targeting vector carrying the DTA (diptheria toxin A) negative selection cassette. The targeting vector was transfected into R1-p.15 cells (129/Sv background) and positive clones were selected using neomycin resistance and injected into C57BL/6 blastocysts. A “cre-deleter” strain (B6.C-Tg(CMV-cre)1Cgn/J; The Jackson Lab. stock n. 006054), expressing the cre recombinase under the cytomegalovirus (CMV) promoter was used to remove the neomycin selection cassette. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Cholinergic striatal neurons are increased in HSAN V homozygous mice despite reduced NGF bioavailability.;Testa Giovanna, Calvello Mariantonietta, Cattaneo Antonino, Capsoni Simona, ;2019;Biochemical and biophysical research communications;509;763-766; 30612733
- The NGFR100W Mutation Specifically Impairs Nociception without Affecting Cognitive Performance in a Mouse Model of Hereditary Sensory and Autonomic Neuropathy Type V.;Testa Giovanna, Mainardi Marco, Morelli Chiara, Olimpico Francesco, Pancrazi Laura, Petrella Carla, Severini Cinzia, Florio Rita, Malerba Francesca, Stefanov Antonia, Strettoi Enrica, Brandi Rossella, Arisi Ivan, Heppenstall Paul, Costa Mario, Capsoni Simona, Cattaneo Antonino, ;2019;The Journal of neuroscience : the official journal of the Society for Neuroscience;39;9702-9715; 31685654