B6;129S2-Esm1tm1.1Ics/Ics
| Status | Available to order |
| EMMA ID | EM:15812 |
| Citation information | RRID:IMSR_EM:15812 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129S2-Esm1tm1.1Ics/Ics |
| Alternative name | Esm1tm1.1Ics |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Esm1tm1.1Ics |
| Gene/Transgene symbol | Esm1 |
Information from provider
| Provider | Philippe Lassalle |
| Provider affiliation | Institut Pasteur |
| Genetic information | The knock-out of Esm1 was obtained by homologous recombination in 129/SvPas in-house derived ES cells (P1 ES cell line). Esm1 exon 1 (ENSMUSE00000404404) and exon 2 (ENSMUSE00000307442) were deleted and replaced by a floxed NeoR cassette. The NeoR cassette was excised by breeding the chimeras with cre recombinase deleter females. The cre transgene was segregated by breeding with C57BL/6N wild-type animals. |
| Phenotypic information | Homozygous:Constitutive deletion of Esm1 in diabetic kidney disease (DKD)-resistant mice modestly increases the degree of diabetes-induced albuminuria versus wild-type controls. By glomerular RNAseq, Esm1 attenuates expression of kidney disease-promoting and interferon (IFN)-related genes, including Ackr2 and Cxcl11.Heterozygous:No data |
| Breeding history | ES cells were 129/SvPas, chimeras were bred with cre deleter in a C57BL/6N genetic background. Current mixed background: 87.5% C57BL/6NTac, 6.25% C57BL/6J, 1.9% C57BL/6, 6.25% 129/SvPas. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France |
Literature references
- Endothelial Cell-Specific Molecule-1 Inhibits Albuminuria in Diabetic Mice.;Zheng Xiaoyi, Higdon Lauren, Gaudet Alexandre, Shah Manav, Balistieri Angela, Li Catherine, Nadai Patricia, Palaniappan Latha, Yang Xiaoping, Santo Briana, Ginley Brandon, Wang Xiaoxin X, Myakala Komuraiah, Nallagatla Pratima, Levi Moshe, Sarder Pinaki, Rosenberg Avi, Maltzman Jonathan S, de Freitas Caires Nathalie, Bhalla Vivek, ;2022;Kidney360;3;2059-2076; 36591362