IMPC phenotypes (gene matching)
C57BL/6-Il1atm1c(EUCOMM)Wtsi/H
| Status | Available to order |
| EMMA ID | EM:15848 |
| Citation information | RRID:IMSR_EM:15848 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Il1atm1c(EUCOMM)Wtsi/H |
| Alternative name | interleukin-1 alpha conditional knockout |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Il1atm1c(EUCOMM)Wtsi |
| Gene/Transgene symbol | Il1a |
Information from provider
| Provider | Emmanuel Pinteaux |
| Provider affiliation | University of Manchester |
| Genetic information | Il1atm1a(EUCOMM)Wtsi embryonic stem cells were purchased from the European Mouse Mutant Cell Repository (EuMMCR). Cells from clone EPD0822-4-H02 were cultured in KO-DMEM + KOSR medium + 2i (MEK inhibitor and GSK3 inhibitor) on a gelatin-coated cell culture dish maintained under standard culture conditions (37°C, 5% CO2, humidified). Cells were then microinjected into 4–8 cell B6N-Tyrc–Brd/BrdCrCrl embryos. Surviving embryos were surgically implanted into the oviduct of day 0.5 postcoitum pseudopregnant mice. Resulting black/white C57BL/6N chimeras were backcrossed onto C57BL/6N wild-type mice to assess germline penetrance. Potential founder mice were screened by PCR for LacZ, Neo, and LoxP sites. This line was further crossed with C57BL/6N-Tg(CAG-Flpo)1Afst/Mmucd mice. The FLP recombinase expression provided by this line resulted in a “conditional ready” (floxed) Il1atm1c(EUCOMM)Wtsi allele, in which exon 4 is flanked by loxP sites. |
| Phenotypic information | Homozygous:No phenotype, as wild-typeHeterozygous:No phenotype, as wild-type |
| Breeding history | Breeding as homozygous in standard cages |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
Literature references
- Fibroblast-specific deletion of interleukin-1 receptor-1 reduces adverse cardiac remodeling following myocardial infarction.;Bageghni Sumia A, Hemmings Karen E, Yuldasheva Nadira Y, Maqbool Azhar, Gamboa-Esteves Filomena O, Humphreys Neil E, Jackson Maj Simonsen, Denton Christopher P, Francis Sheila, Porter Karen E, Ainscough Justin Fx, Pinteaux Emmanuel, Drinkhill Mark J, Turner Neil A, ;2019;JCI insight;5;; 31393855