C57BL/6J-Proser1em1Doca/H
| Status | Available to order |
| EMMA ID | EM:15854 |
| Citation information | RRID:IMSR_EM:15854 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6J-Proser1em1Doca/H |
| Alternative name | Proser1 KO |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Proser1em1Doca |
| Gene/Transgene symbol | Proser1 |
Information from provider
| Provider | Kasper Rasmussen |
| Provider affiliation | Division of Molecular, Cellular, and Developmental Biology (MCDB), University of Dundee |
| Genetic information | The Proser1 knockout allele was generated by injecting fertilised 1-cell zygotes from C57BL/6J mice with Cas9 mRNA and and a small guide RNA (5’- GTGCTGGATGAAATTCGAA-3’) targeting exon 1 of Proser1 (ENSMUSG00000049504). Injected zygotes were cultured to blastocyst stage and implanted into pseudo-pregnant females. Genomic DNA harvested from offspring was screened for the presence of out-of-frame indels. Founder animals harboring an allele with a disruptive 7 bp deletion starting at position 53,371,732 of chromosome 3 (Build GRCm39) were selected for backcrossing to C57BL/6J animals and further breeding. |
| Phenotypic information | Homozygous:Homozygous mice display prenatal lethality with approximately 50% of KO offspring surviving to adulthood. Adult mice weigh less than their wildtype counterparts, and have reduced cranial volume. A proportion of adult KO animals display a syndromic phenotype characterised by frequent eye abnormalities, including microphthalmia, anopthalmia, and cataracts, as well as intermittent tremors and failure to thrive.Heterozygous:No obvious phenotype |
| Breeding history | Animals carrying the Proser1 knockout allele has been backcrossed to C57BL/6J (Charles River strain code 632) for 7 generations. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- PROSER1 modulates DNA demethylation through dual mechanisms to prevent syndromic developmental malformations.;Fleming Anna, Knatko Elena V, Li Xiang, Zoch Ansgar, Heckhausen Zoe, Stransky Stephanie, Brenes Alejandro J, Sidoli Simone, Hajkova Petra, O'Carroll Dónal, Rasmussen Kasper D, ;2024;Genes & development;38;952-964; 39562138