C57BL/6N-C9orf72em2.2Tjcu/H

Status

Available to order

EMMA IDEM:15855
Citation informationRRID:IMSR_EM:15855 

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International strain nameC57BL/6N-C9orf72em2.2Tjcu/H
Alternative nameC57BL/6N-C9orf72 em2.2Tjcu/H
Strain typeEndonuclease-mediated
Allele/Transgene symbolC9orf72em2.2Tjcu
Gene/Transgene symbolC9orf72

Information from provider

ProviderThomas Cunningham
Provider affiliationInstitute of Prion Diseases, University College London
Genetic informationThis strain carries a C9orf72 allele where the C9orf72 gene is humanised from the start of intron 1 through to the ATG start codon in exon 2. All mouse promotor sequences and UTRs have been retained. The allele also carries a large repeat expansion within the humanised intron 1. The targeting construct for C9orf72 humanisation was developed by the MMoN group at MRC Harwell. This construct was used by the Genome Engineering Core at the Wellcome Centre Human Genetics, Oxford as an HDR template for CRISPR editing in C57BL/6N ES cells.
Phenotypic informationHomozygous:
Not determined

Heterozygous:
Not determined
Breeding historyThe F0 founder (derived from C57BL/6N ES cells) was first bred 3 generations to C57BL/6N wild type stock. F3 animals were then bred to transgenic mice expressing PiggyBac transposase (maintained on a C57BL/6N background) to remove the selection cassette, and crossed a further generation to C57BL/6N wild type stock.
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • epithelial hyperplasia / IMPC
  • hyperplasia / IMPC
  • decreased total body fat amount / IMPC
  • enlarged spleen / IMPC
  • increased circulating alkaline phosphatase level / IMPC
  • chronic inflammation / IMPC
  • abnormal spleen morphology / IMPC
  • lymphoid hyperplasia / IMPC
  • abnormal vertebral arch morphology / IMPC
  • increased lean body mass / IMPC
  • increased spleen weight / IMPC
  • myeloid hyperplasia / IMPC
  • inflammation / IMPC
  • small testis / IMPC
  • enlarged lymph nodes / IMPC
  • acute inflammation / IMPC

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

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Practical information

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