B6;129-Arctm1.1Grnt/WtsiH
| Status | Available to order |
| EMMA ID | EM:15858 |
| Citation information | RRID:IMSR_EM:15858 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129-Arctm1.1Grnt/WtsiH |
| Alternative name | B6;129-Arc |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Arctm1.1Grnt |
| Gene/Transgene symbol | Arc |
Information from provider
| Provider | Seth Grant |
| Provider affiliation | Wellcome Trust Sanger Institute |
| Genetic information | Information sourced from Document S1. Supplemental Experimental Procedures and Figure S1 (https://doi.org/10.1016/j.celrep.2017.09.045): This strain carries a knock in of a sequence coding for a TAP (Tandem Affinity Purification) tag (containing Histidine Affinity Tag (HAT), TEV protease and 3x Flag sequences), followed by a loxP site flanked neomycin resistance gene cassette, inserted before the translation stop codon in exon 1. The neo cassette was removed through subsequent cre-mediated recombination. This leaves an allele coding for the endogenous protein with a C-terminal TAP tag. No change in expression of the targeted gene was observed. |
| Breeding history | Maintained by backcross on CBLJ (C57BL/6JIco). Without neo. Unsure on viability or fertility (if Hom would have been produced) |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | not known |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Familial cortical myoclonus / Orphanet_319189
Literature references
- Arc Requires PSD95 for Assembly into Postsynaptic Complexes Involved with Neural Dysfunction and Intelligence.;Fernández Esperanza, Collins Mark O, Frank René A W, Zhu Fei, Kopanitsa Maksym V, Nithianantharajah Jess, Lemprière Sarah A, Fricker David, Elsegood Kathryn A, McLaughlin Catherine L, Croning Mike D R, Mclean Colin, Armstrong J Douglas, Hill W David, Deary Ian J, Cencelli Giulia, Bagni Claudia, Fromer Menachem, Purcell Shaun M, Pocklington Andrew J, Choudhary Jyoti S, Komiyama Noboru H, Grant Seth G N, ;2017;Cell reports;21;679-691; 29045836