B6J(B6CBA)-Kitlem2Cnbc/Cnbc
| Status | Available to order |
| EMMA ID | EM:15919 |
| Citation information | RRID:IMSR_EM:15919 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6J(B6CBA)-Kitlem2Cnbc/Cnbc |
| Alternative name | Kitl |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Kitlem2Cnbc |
| Gene/Transgene symbol | Kitl |
Information from provider
| Provider | Lluis Montoliu |
| Provider affiliation | CNB-CSIC |
| Genetic information | This mutation is a knockin of a human mutation into the mouse Kitl gene. c.200_202del; p.His67_68delinsArg, see PMID: 26522471. It was generated in Madrid at CNB-CSIC and has been used in cross to a C57BL/6N Cdh23Ahl+ genetic background, carrying the repaired allele of Cdh23ahl. |
| Phenotypic information | Homozygous:The homozygotes are lethal.Heterozygous:The humans heterozygous for this mutation show deafness which can be asymmetric, but we have not found any deafness in the heterozygous mice, just a belly spot and slightly paler fur. |
| Breeding history | The original knock-in mutation was initially established at CNB by CRISPR-Cas9 genome editing in a B6CBAF2 hybrid mouse background. Founder mice were backcrossed to C57BL/6JOlaHsd for 5 consecutive generations. Current genetic background: C57BL/6J; some residual CBA genetic background might be still present. |
| References | None available |
| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6JOlaHsd males |