C57BL/6-Il1btm1c(EUCOMM)Wtsi/H
| Status | Available to order |
| EMMA ID | EM:15920 |
| Citation information | RRID:IMSR_EM:15920 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Il1btm1c(EUCOMM)Wtsi/H |
| Alternative name | interleukin-1 beta conditional knockout |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Il1btm1c(EUCOMM)Hmgu |
| Gene/Transgene symbol | Il1b |
Information from provider
| Provider | Emmanuel Pinteaux |
| Provider affiliation | University of Manchester |
| Additional owner | EUCOMM ES cells used to produce this strain |
| Genetic information | Il1btm1a(EUCOMM)Hmgu embryonic stem cells were purchased from the European Mouse Mutant Cell Repository (EuMMCR). Cells from clone HEPD0840-8-E03 were cultured in KO-DMEM + KOSR + 2i (MEK inhibitor and GSK3 inhibitor) on a gelatin-coated cell culture dish maintained under standard culture conditions (37°C, 5% CO2, humidified). Cells were then microinjected into 4 to 8 cell B6N-Tyrc-Brd/BrdCrCrl embryos. Surviving embryos were surgically implanted into the oviduct of day 0.5 postcoitum pseudopregnant mice. Resulting black/white C57BL/6N chimeras were back-crossed onto C57BL/6N wild-type mice to assess germline penetrance. Potential founder mice were screened by PCR for LacZ, Neo and LoxP sites. This line was further crossed with C57BL/6N-Tg(CAG-Flpo)1Afst/Mmucd mice. The flp recombinase expression provided by this line resulted in a "conditional ready" (floxed) Il1btm1c(EUCOMM)Hmgu allele, in which exons 4 and 5 are flanked by loxP sites. |
| Phenotypic information | Homozygous:No phenotype, as wild typeHeterozygous:No phenotype, as wild type |
| Breeding history | Breeding as homozygous in standard cages |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Transfer of complex regional pain syndrome to mice via human autoantibodies is mediated by interleukin-1-induced mechanisms.;Helyes Zsuzsanna, Tékus Valéria, Szentes Nikolett, Pohóczky Krisztina, Botz Bálint, Kiss Tamás, Kemény Ágnes, Környei Zsuzsanna, Tóth Krisztina, Lénárt Nikolett, Ábrahám Hajnalka, Pinteaux Emmanuel, Francis Sheila, Sensi Serena, Dénes Ádám, Goebel Andreas, ;2019;Proceedings of the National Academy of Sciences of the United States of America;116;13067-13076; 31182576