C57BL/6J-Brca2em1Nki/NkiOrl

Status

Available to order

EMMA IDEM:15927
Citation informationRRID:IMSR_EM:15927 

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International strain nameC57BL/6J-Brca2em1Nki/NkiOrl
Alternative nameBrca2_T5805del
Strain typeEndonuclease-mediated
Allele/Transgene symbolBrca2em1Nki
Gene/Transgene symbolBrca2

Information from provider

ProviderLinda Henneman
Provider affiliationNKI-AVL
Genetic informationBrca2 gene - frameshift mutation. Deleting nucleotide 5805(T) with CRISPR/Cas9 using gRNA (5'-GGGATTTTTAGCACAGCAAG-3'). In addition, 2 silent mutations are generated: S1932 AGC>TCA and A1934 GCA>GCC. Cas9 protein from IDT was used to generate the strain. No gene encoding Cas9 was inserted into the mouse genome.
Phenotypic informationHomozygous:
Homozygous Brac2_T5805del mice are embryonic lethal.

Heterozygous:
Heterozygous Brca2_T5805del mice are healthy and do not have a phenotype.
Breeding historyThe founder was backcrossed 2 times with C57BL/6J.
ReferencesNone available
Homozygous fertileno
Homozygous viableno
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (gene matching)
  • abnormal rib morphology / MGI
  • abnormal sternum morphology / MGI
  • decreased bone marrow cell number / MGI
  • kinked tail / MGI
  • abnormal branching of the mammary ductal tree / MGI
  • spleen hypoplasia / MGI
  • open neural tube / MGI
  • small ovary / MGI
  • small testis / MGI
  • abnormal epidermal layer morphology / MGI
  • abnormal epidermis stratum basale morphology / MGI
  • abnormal epidermis stratum spinosum morphology / MGI
  • absent epidermis stratum corneum / MGI
  • decreased body weight / MGI
  • impaired hematopoiesis / MGI
  • abnormal embryo development / MGI
  • decreased embryo size / MGI
  • abnormal visceral yolk sac morphology / MGI
  • male infertility / MGI
  • female infertility / MGI
  • neoplasm / MGI
  • increased tumor incidence / MGI
  • increased T cell derived lymphoma incidence / MGI
  • increased carcinoma incidence / MGI
  • decreased tumor incidence / MGI
  • abnormal skin morphology / MGI
  • abnormal motor capabilities/coordination/movement / MGI
  • premature death / MGI
  • abnormal developmental patterning / MGI
  • abnormal embryonic tissue morphology / MGI
  • abnormal extraembryonic tissue morphology / MGI
  • abnormal axial skeleton morphology / MGI
  • no abnormal phenotype detected / MGI
  • abnormal hematopoietic system morphology/development / MGI
  • absent ovarian follicles / MGI
  • abnormal chromosome morphology / MGI
  • embryonic growth retardation / MGI
  • increased mortality induced by ionizing radiation / MGI
  • abnormal mitosis / MGI
  • fetal growth retardation / MGI
  • decreased incidence of tumors by chemical induction / MGI
  • enlarged allantois / MGI
  • absent germ cells / MGI
  • increased thymus weight / MGI
  • abnormal amnion morphology / MGI
  • sepsis / MGI
  • azoospermia / MGI
  • growth/size/body region phenotype / MGI
  • reproductive system phenotype / MGI
  • abnormal cell physiology / MGI
  • increased apoptosis / MGI
  • early cellular replicative senescence / MGI
  • abnormal DNA repair / MGI
  • decreased survivor rate / MGI
  • decreased common myeloid progenitor cell number / MGI
  • chromosomal instability / MGI
  • increased sensitivity to induced cell death / MGI
  • adrenal gland hyperplasia / MGI
  • decreased fetal weight / MGI
  • decreased birth weight / MGI
  • increased mammary gland tumor incidence / MGI
  • decreased tumor latency / MGI
  • abnormal double-strand DNA break repair / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • neonatal lethality, incomplete penetrance / MGI
  • perinatal lethality, incomplete penetrance / MGI
  • prenatal lethality, complete penetrance / MGI
  • embryonic lethality, complete penetrance / MGI
  • embryonic lethality between somite formation and embryo turning, complete penetrance / MGI
  • embryonic lethality during organogenesis, complete penetrance / MGI
  • prenatal lethality, incomplete penetrance / MGI
  • embryonic lethality during organogenesis, incomplete penetrance / MGI
  • lethality throughout fetal growth and development, incomplete penetrance / MGI
  • abnormal proamniotic cavity morphology / MGI
  • decreased fibroblast proliferation / MGI
  • increased lymphoma incidence / MGI
  • ovary degeneration / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

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Legally binding conditions for the transfer

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