C57BL/6-Lgr5^{em1(cre/ERT2)Kba}/Orl

Status	Under development - register interest
EMMA ID	EM:15959
Citation information	RRID:IMSR_EM:15959 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6-Lgr5^{em1(cre/ERT2)Kba}/Orl
Alternative name	Lgr5em1(cre/ERT2)Kba
Strain type	Endonuclease-mediated
Allele/Transgene symbol	Lgr5^{em1(cre/ERT2)Kba}
Gene/Transgene symbol	Lgr5

Information from provider

Provider	Konrad Basler
Provider affiliation	DMLS, University of Zurich
Genetic information	Non-variegated cre-ERT2 driver and reporter of Lgr5 (Gpr49) expression. CRISPR targeting introduced a sequence containing an Lgr5-2A-CreERT2-2A-mOrange2 construct at 3' end of the Lgr5 gene. CRISPR technology details: pronuclear microinjection of Cas9 protein loaded gRNA and the plasmid harbouring the homology directed repair (HDR) template in fertilized zygotes.
Phenotypic information	Homozygous: none Heterozygous: none
Breeding history	The pups which inherited the targeted allele were crossed with C57BL/6J for more than 15 generations.
References	Tracing colonic embryonic transcriptional profiles and their reactivation upon intestinal damage.;Fazilaty Hassan, Brügger Michael David, Valenta Tomas, Szczerba Barbara M, Berkova Linda, Doumpas Nikolaos, Hausmann George, Scharl Michael, Basler Konrad, ;2021;Cell reports;36;109484; 34348153
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	homozygous C57BL/6J males

Disease and phenotype information

IMPC phenotypes (gene matching)

increased circulating bilirubin level / IMPC
increased CD4-negative, CD25-positive NK T cell number / IMPC
preweaning lethality, complete penetrance / IMPC
increased CD8-positive, CD25-positive alpha-beta T cell number / IMPC
increased CD11b-high dendritic cell number / IMPC
no spontaneous movement / IMPC
increased grip strength / IMPC
cleft palate / IMPC

Literature references

Tracing colonic embryonic transcriptional profiles and their reactivation upon intestinal damage.;Fazilaty Hassan, Brügger Michael David, Valenta Tomas, Szczerba Barbara M, Berkova Linda, Doumpas Nikolaos, Hausmann George, Scharl Michael, Basler Konrad, ;2021;Cell reports;36;109484; 34348153

Information on how we integrate external resources can be found here

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6-Lgr5em1(cre/ERT2)Kba/Orl