| Status | Under development - register interest |
| EMMA ID | EM:15966 |
| Citation information | RRID:IMSR_EM:15966 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129-Chordc1tm1Mbra/Cnrm |
| Alternative name | chordc1+/- (morgana +/-) C57BL/6j × 129SV |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Chordc1tm1Mbra |
| Gene/Transgene symbol | Chordc1 |
| Provider | Mara Brancaccio |
| Provider affiliation | Department of Molecular Biotechnology and Health Sciences, University of Torino |
| Genetic information | The Morgana coding gene (Chordc1) was inactivated by homologous recombination in mouse ES cells. The knock-out construct consisted of a 5′ arm (a 3.5 kb genomic fragment), a β-galactosidase gene with a neomycin resistance cassette (6 kb) and a 3′ arm (a 6.3 kb genomic fragment). ES recombinant clones lacking a 30 bp sequence including the Chordc1 ATG translation start site were identified by Southern blot analysis and injected into C57BL/6 blastocysts to generate chimeras (Ferretti R et al., Dev Cell. 2010, 18(3):486-95. doi: 10.1016/j.devcel.2009.12.020. PMID:20230755) |
| Phenotypic information | Homozygous:Homozygous mutation of the Morgana coding gene (Chordc1) leads to preimplantation lethality. At 3.5 days post coitum, morphologically normal Chordc1-/⁻- embryos were observed at a Mendelian frequency, but no embryos were detectable after implantation.Heterozygous:Chordc1+/- (Morgana+/-) mice exhibit increased susceptibility to neoplastic transformation when treated at birth with the carcinogen 7,12-dimethylbenz[a]anthracene (DMBA) and examined five months later for lung tumor formation. Morgana+/− mice showed a 2.5-fold increase in lung tumor frequency compared to their wild-type littermates subjected to the same treatment. Starting at 12 months of age, 60% of Chordc1+/- (Morgana+/- ) mice spontaneously develop a lethal myeloproliferative disorder resembling human atypical chronic myeloid leukemia (aCML), characterized by centrosome amplification and cytogenetic abnormalities in the bone marrow. |
| Breeding history | ES recombinant clones (129Sv) have been injected into C57BL/6 blastocysts to generate chimeras. Chimeras have been mated with C57BL/6J and offspring of different chimeras were mated together. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
