B6.CB-Dmrt2em1Cnbbm/Cnbc
| Status | Available to order |
| EMMA ID | EM:15970 |
| Citation information | RRID:IMSR_EM:15970 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.CB-Dmrt2em1Cnbbm/Cnbc |
| Alternative name | B6CBA-Dmrt2em2/Cnbbm |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Dmrt2em1Cnbbm |
| Gene/Transgene symbol | Dmrt2 |
Information from provider
| Provider | Esther Serrano-Saiz |
| Provider affiliation | CBM-CSIC |
| Genetic information | Two loxP sites have been introduced into exon 2 (including the translation start codon) of the Dmrt2 gene. The line needs to be crossed with a cre recombinase-expressing strain in order to generate a Dmrt2 mutant. This conditional mutant will allow the functional study of Dmrt2, including in postnatal stages of development. The sequence between the two loxP sites has been codon optimized but without generating aminoacid changes. CRISPR technology details: The construct was generated by easi-Crispr. The Alt-R S.p. HiFi Cas9 Nuclease was obtained from IDT (Integrated DNA Technologies) and Cas9 was not inserted into the mouse genome. |
| Phenotypic information | Homozygous:No phenotype has been observedHeterozygous:No phenotype has been observed |
| Breeding history | Originally produced in C57BL/6 x CBA zygotes; 5 crosses with C57BL/6. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6J males |