C57BL/6-Ubn2^em1Awu/Orl

Status	Under development - register interest
EMMA ID	EM:15974
Citation information	RRID:IMSR_EM:15974 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6-Ubn2^em1Awu/Orl
Alternative name	Ubn2 +/-
Strain type	Endonuclease-mediated
Allele/Transgene symbol	Ubn2^em1Awu
Gene/Transgene symbol	Ubn2

Information from provider

Provider	Anton Wutz
Provider affiliation	ETH Zürich
Genetic information	A 17 kb deletion of the ubinuclein 2 (Ubn2) gene was introduced using CRISPR/Cas9 pronuclear microinjection into zygotes. The deletion spans exons 3-7. CRISPR technology details: the deletion was introduced by cutting the Ubn2 expression vector with two Cas9 RNPs.
Phenotypic information	Homozygous: We have observed that homozygous Ubn2 mutant males are healthy and fertile, though they are born at a submendelian ratio. In females, homozygosity is mostly embryonic lethal before day 12.5 in the C57BL/6 genetic background, though there is some variability in the phenotype. Rarely, homozygous mutant female are born, but they are infertile. The Ubn1 and Ubn2 mutations contribute additively, whereby the combined mutation of Ubn1 (EMMA strain EM:15975) and Ubn2 (this strain) approximates the Hira (histone cell cycle regulator) lethality which could be expected from the molecular function of both genes. Heterozygous: Heterozygous males and females are healthy and fertile.
Breeding history	Mutation was introduced into C57BL/6J background strain. The mutation is maintained in heterozygous state.
References	Ubinuclein 2 is essential for mouse development and functions in X chromosome inactivation.;Monfort Asun, Di Minin Giulio, Sting Sarah, Dumeau Charles Etienne, Scambler Peter, Wutz Anton, ;2025;PLoS genetics;21;e1011711; 40455860
Homozygous fertile	males only
Homozygous viable	males only
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France
Animals used for archiving	heterozygous C57BL/6J males

Disease and phenotype information

IMPC phenotypes (gene matching)

abnormal head size / IMPC
abnormal head shape / IMPC
pallor / IMPC
abnormal facial morphology / IMPC
apoptosis / IMPC
decreased mean corpuscular hemoglobin concentration / IMPC
abnormal embryo size / IMPC
protruding tongue / IMPC
spermatogenesis defect / IMPC
male infertility / IMPC
preweaning lethality, incomplete penetrance / IMPC
abnormal eyelid fusion / IMPC
exencephaly / IMPC
anophthalmia / IMPC
decreased mean corpuscular hemoglobin / IMPC
decreased exploration in new environment / IMPC

Literature references

Ubinuclein 2 is essential for mouse development and functions in X chromosome inactivation.;Monfort Asun, Di Minin Giulio, Sting Sarah, Dumeau Charles Etienne, Scambler Peter, Wutz Anton, ;2025;PLoS genetics;21;e1011711; 40455860

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6-Ubn2em1Awu/Orl