IMPC phenotypes (gene matching)
C57BL/6-Ubn1em1Awu/Orl
| Status | Under development - register interest |
| EMMA ID | EM:15975 |
| Citation information | RRID:IMSR_EM:15975 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Ubn1em1Awu/Orl |
| Alternative name | Ubn1 -/- |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Ubn1em1Awu |
| Gene/Transgene symbol | Ubn1 |
Information from provider
| Provider | Anton Wutz |
| Provider affiliation | ETH Zürich |
| Genetic information | A 10 kb deletion in the ubinuclein 1 (Ubn1) gene, spanning exons 3-7, was introduced. CRISPR technology details: the deletion was introduced by cutting the Ubn2 expression vector with two Cas12a RNPs. |
| Phenotypic information | Homozygous:Homozygous Ubn1 mutant mice are healthy and fertile. We have observed no phenotype of the mutation. The Ubn1 and Ubn2 mutations contribute additively, whereby the combined mutation of Ubn1 (this strain) and Ubn2 (EMMA strain EM:15974) approximates the Hira (histone cell cycle regulator) lethality which could be expected from the molecular function of both genes.Heterozygous:Heterozygous Ubn1 mutant mice are healthy and fertile. |
| Breeding history | Mutation was introduced into C57BL/6J background strain by zygote electroporation. The mutation is maintained in a homozygous state. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | homozygous C57BL/6J males |
Disease and phenotype information
Literature references
- Ubinuclein 2 is essential for mouse development and functions in X chromosome inactivation.;Monfort Asun, Di Minin Giulio, Sting Sarah, Dumeau Charles Etienne, Scambler Peter, Wutz Anton, ;2025;PLoS genetics;21;e1011711; 40455860
- Introducing gene deletions by mouse zygote electroporation of Cas12a/Cpf1.;Dumeau Charles-Etienne, Monfort Asun, Kissling Lucas, Swarts Daan C, Jinek Martin, Wutz Anton, ;2019;Transgenic research;28;525-535; 31482512