C57BL/6NTac-Tecta^tm6Gpr/H

Status	Available to order
EMMA ID	EM:15986
Citation information	RRID:IMSR_EM:15986 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6NTac-Tecta^tm6Gpr/H
Alternative name	Tecta-tm6Gpr
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Tecta^tm6Gpr
Gene/Transgene symbol	Tecta

Information from provider

Provider	Guy Richardson
Provider affiliation	University of Sussex
Genetic information	A targeting vector that was designed and constructed by Vector Biolabs (Eagleville PA, USA) for deleting Tecta and expressing a Tectb-IRES-Egfp minigene under the control of the endogenous Tecta promotor was generated using a combination of PCR and conventional cloning techniques. The vector consisted of a 2832 bp left arm with the ATG start codon of the Tecta open reading frame (ORF) at the 3’ end fused via a PmeI linker to the Tectb ORF, followed by an IRES, the Egfp ORF, an SV40 polyadenylation signal sequence, a neomycin resistance cassette flanked with loxP sites, a 4903 bp right arm from the Tecta gene beginning 314 bp 3’ of exon 2 and a thymidine kinase cassette. The Tecta arms were prepared from a 129SvEvBrd genomic DNA clone as described previously (DOI: 10.1016/s0896-6273(00)00102-1). For the generation of transgenic mouse line Tectatm6Gpr, embryonic stem (ES) cells were transfected with I-CeuI linearised targeting vector and resistant colonies selected as described (DOI: 10.1016/s0896-6273(00)00102-1). Individual colonies were picked and screened by Southern blotting and correctly targeted clones identified. Transgenic mice were prepared by microinjection of mouse blastocysts and a chimeric male founder was crossed to a wild type S129SvEv female. Offspring carrying the insertion were then crossed with a beta-actin Cre line to remove the floxed neomycin selection cassette and, once it had been established that the selection cassette had been deleted, the offspring were characterized. Additional information are in the following publication (DOI: 10.1113/JP280905).
Phenotypic information	Homozygous: Highly elevated hearing threshold Heterozygous: normal
Breeding history	Breed for more than 10 generations on the above background
References	A targeted deletion in alpha-tectorin reveals that the tectorial membrane is required for the gain and timing of cochlear feedback.;Legan P K, Lukashkina V A, Goodyear R J, Kössi M, Russell I J, Richardson G P, ;2000;Neuron;28;273-85; 11087000 MET currents and otoacoustic emissions from mice with a detached tectorial membrane indicate the extracellular matrix regulates Ca²⁺ near stereocilia.;Jeng Jing-Yi, Harasztosi Csaba, Carlton Adam J, Corns Laura F, Marchetta Philine, Johnson Stuart L, Goodyear Richard J, Legan Kevin P, Rüttiger Lukas, Richardson Guy P, Marcotti Walter, ;2021;The Journal of physiology;599;2015-2036; 33559882
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Autosomal recessive non-syndromic sensorineural deafness type DFNB / Orphanet_90636
Autosomal dominant non-syndromic sensorineural deafness type DFNA / Orphanet_90635

MGI phenotypes (gene matching)

audiogenic seizures / MGI
abnormal hearing physiology / MGI
abnormal otolithic membrane morphology / MGI
no phenotypic analysis / MGI
enlarged otoliths / MGI
decreased otolith number / MGI
abnormal tectorial membrane morphology / MGI
detached tectorial membrane / MGI
abnormal cochlear microphonics / MGI
decreased cochlear microphonics / MGI
decreased cochlear nerve compound action potential / MGI
abnormal hair cell mechanoelectric transduction / MGI
abnormal cochlear outer hair cell physiology / MGI
abnormal cochlear hair cell stereociliary bundle morphology / MGI
abnormal distortion product otoacoustic emission / MGI
absent distortion product otoacoustic emissions / MGI
hearing/vestibular/ear phenotype / MGI
behavior/neurological phenotype / MGI
impaired hearing / MGI
abnormal otoacoustic response / MGI
increased or absent threshold for auditory brainstem response / MGI
abnormal Hensen stripe morphology / MGI
absent Hensen stripe / MGI
abnormal Kimura membrane morphology / MGI
abnormal tectorial membrane marginal band morphology / MGI
abnormal tectorial membrane covernet morphology / MGI
abnormal tectorial membrane striated-sheet matrix morphology / MGI

Literature references

A targeted deletion in alpha-tectorin reveals that the tectorial membrane is required for the gain and timing of cochlear feedback.;Legan P K, Lukashkina V A, Goodyear R J, Kössi M, Russell I J, Richardson G P, ;2000;Neuron;28;273-85; 11087000
MET currents and otoacoustic emissions from mice with a detached tectorial membrane indicate the extracellular matrix regulates Ca²⁺ near stereocilia.;Jeng Jing-Yi, Harasztosi Csaba, Carlton Adam J, Corns Laura F, Marchetta Philine, Johnson Stuart L, Goodyear Richard J, Legan Kevin P, Rüttiger Lukas, Richardson Guy P, Marcotti Walter, ;2021;The Journal of physiology;599;2015-2036; 33559882

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Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

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C57BL/6NTac-Tectatm6Gpr/H