Current name: Humamice 4 ; International name: Rag2tm1Fwa B2mtm1Unc H2-Ab1tm1Doi Il2rgtm1Sug Tg(HLA-A/H2-D/B2M)1Bpe Tg(HLA-DRA*0101,HLA-DRB1*0101)1Dma/Orl,Tg(SIRPA)1Flv
| Status | Under development - register interest |
| EMMA ID | EM:16001 |
| Citation information | RRID:IMSR_EM:16001 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | Current name: Humamice 4 ; International name: Rag2tm1Fwa B2mtm1Unc H2-Ab1tm1Doi Il2rgtm1Sug Tg(HLA-A/H2-D/B2M)1Bpe Tg(HLA-DRA*0101,HLA-DRB1*0101)1Dma/Orl,Tg(SIRPA)1Flv |
| Alternative name | Current name: Humamice 4 ; International name: Rag2tm1Fwa B2mtm1Unc H2-Ab1tm1Doi Il2rgtm1Sug Tg(HLA- |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(SIRPA)1Flv |
| Gene/Transgene symbol | Tg(SIRPA)1Flv |
Information from provider
| Provider | Yu Chun Lone |
| Provider affiliation | INSERM U1197 |
| Genetic information | Humamice 1 line (EMMA strain ID EM:06326) + hSIRPA transgene (the endogenous mouse Sirpa gene has been replaced by the human SIRPA gene, the expression of hSIRPA is under the mSirpa promoter). 7 genetic modifications : 4 KO genes (RAG2, IAbeta, beta2m, IL2rg genes) + 3 transgenes (HLAA2, HLADRA-DRB1, hSIRPA). |
| Phenotypic information | Homozygous:Immunodeficient line.Heterozygous:Possibly immunodeficient line. |
| Breeding history | The Humamice 4 mice on the BALB/c background were transferred to the CNRS TAAM unit from the Sorbonne University MSU 028 unit in 2021. The line has been backcrossed at the TAAM with Humamice 1 strain (EM:06326) on the C57BL/6 background for at least 6 generations. This was followed by several generations of intercross: Humamice 1 hSirpa Tg x Humamice 1 hSirpa Tg. The Humamice 1 modifications have been established in homozygous form. The hSirpa modification has not been established in homozygous form. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | yes |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |