IMPC phenotypes (gene matching)
- increased circulating lipase level / IMPC
Oma1E324Q/E324Q
| Status | Under development - register interest |
| EMMA ID | EM:16048 |
| Citation information | RRID:IMSR_EM:16048 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | Oma1E324Q/E324Q |
| Alternative name | Oma1E324Q/E324Q |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Oma1 |
| Gene/Transgene symbol | Oma1 |
Information from provider
| Provider | Timothy Wai |
| Provider affiliation | Institut Pasteur |
| Genetic information | We generated a single point mutation impairing the catalytic activity of the mitochondrial protease OMA1. This was done by CRISPR/CAS9 genomic editing. Details of the creation of this mouse can be found here: https://www.biorxiv.org/content/10.1101/2025.08.18.668848v1. For transgenesis, pronuclei of fertilized embryos were co-microinjected with RNP CRISPR/Cas9 complex (20 ng/μL Cas9 protein, 20 ng/ μL sgRNA) together with 20 ng/μl of ssODN (all reagents from Integrated DNA Technologies, IDT). |
| Phenotypic information | Homozygous:No observable phenotype of homozgyous mice. Cardiac mitochondrial proteomes were performed in https://www.biorxiv.org/content/10.1101/2025.08.18.668848v1. Mutant mice are resistant to the mitochondrial stress-induced activation of the integrated stress response.Heterozygous:Not studied. |
| Breeding history | Founder carrying point mutation was outcrossed to C57BL/6NCrl for 3 generations and mice are now maintained by heterozygous intercross. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6NCrl males |