IMPC phenotypes (gene matching)
Spi1tm1(EGFP/cre/ERT2)Wtsi/Ics
| Status | Available to order |
| EMMA ID | EM:16074 |
| Citation information | RRID:IMSR_EM:16074 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | Spi1tm1(EGFP/cre/ERT2)Wtsi/Ics |
| Alternative name | Spi1tm1(EGFP/cre/ERT2)Wtsi/Ics |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Spi1tm1(EGFP/cre/ERT2)Wtsi |
| Gene/Transgene symbol | Spi1 |
Information from provider
| Provider | ICS, Institut Clinique de la Souris |
| Provider affiliation | ICS, Institut Clinique de la Souris |
| Genetic information | The line was generated from the CEPD0033_2_A04 EUCOMMtools ES cell clone, which was validated by Southern blot. The following elements were inserted into intron 3: FRT site, En2 splice acceptor sequence, sequence coding for F2A self-cleaving peptide, EGFP reporter gene, sequence coding for T2A self-cleaving peptide, cre/ERT2 inducible recombinase gene, SV40 poly(A) signal sequence, Rox site-flanked puromycin resistance gene cassette, and a loxP site. A second loxP site was inserted into intron 4. This creates a reporter-recombinase knockout allele with a floxed exon 4. |
| Phenotypic information | Homozygous:N/AHeterozygous:N/A |
| Breeding history | Pure C57BL/6N genetic background. Current background: C57BL/6NCrl 50%, C57BL/6NTac 50% |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France |