Adcy5em1(R419W)
| Status | Available to order |
| EMMA ID | EM:16082 |
| Citation information | RRID:IMSR_EM:16082 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | Adcy5em1(R419W) |
| Alternative name | Adcy5em1(R419W) |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Adcy5 |
| Gene/Transgene symbol | Adcy5 |
Information from provider
| Provider | Emmanuel Roze |
| Provider affiliation | service de neurologie / troubles du mouvement, AP‑HP Sorbonne Université |
| Genetic information | The Adcy5supR419W/sup mouse line was generated on a C57BL/6N background using CRISPR/Cas9-mediated genome editing at the Institut Clinique de la Souris – PHENOMIN (http://www.phenomin.fr). Mutations were introduced into exon 2 of the Adcy5 gene (ENSMUSG00000022840), located on chromosome 16. Four mutations were simultaneously introduced into wild-type C57BL/6N embryos (Extended Data Fig. 5a). A CRISPR ribonucleoprotein complex—comprising a crRNA (5′-GCCGAGCCTGGATACACTCC-3′; MIT score: 88, as predicted by the CRISPOR website), tracrRNA, and wild-type Cas9—was co-electroporated with a 128-nucleotide single-stranded donor DNA into fertilized C57BL/6N oocytes. Resulting founders were selected based on genotyping and confirmed by Sanger sequencing. Among these, the c.1255C |
| Phenotypic information | Homozygous:Heterozygous Adcy5 mutant mice from the selected founder line were backcrossed to the C57BL/6N background for at least 10 generations. Genotype distribution among Adcy5 littermates followed Mendelian ratios, and no lethality was observed in homozygous or heterozygous mutants during embryogenesis, at birth, or in the pre-weaning period.Heterozygous:see doi: 10.2139/ssrn.5399781 |
| Breeding history | The founder was bred with C57BL/6N wild type mouse. All backcrosses were performed on C57BL/6N genetic background and the sperm from F3 heterozygous animals was cryopreserved |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France |