B6J.129-Arhgap15tm1Ehi/Cnrm
| Status | Under development - register interest |
| EMMA ID | EM:16172 |
| Citation information | RRID:IMSR_EM:16172 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6J.129-Arhgap15tm1Ehi/Cnrm |
| Alternative name | ArhGAP15 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Arhgap15tm1Ehi |
| Gene/Transgene symbol | Arhgap15 |
Information from provider
| Provider | Angela Della Sala |
| Provider affiliation | university of Turin |
| Genetic information | The Arhgap15 gene-targeting vector disrupted the first coding exon by inserting a lacZ gene and neomycin resistance cassette in a mouse ES cell line (129Sv). Properly targeted clones were identified by southern blot, and subsequently injected into C57BL/6J blastocytes. Two of three recombinant clones were used to generate germ line-transmitting chimeras. |
| Phenotypic information | Homozygous:Homozygous animals display a clear and fully penetrant phenotype consistent with the targeted genetic modification. They remain viable and fertile, and no severe developmental defects or welfare-impacting abnormalities have been observed under normal housing conditions. Any physiological or molecular alterations associated with the mutation are stable and do not interfere with breeding, general health, or long-term colony maintenance.Heterozygous:Heterozygous (or hemizygous) mice show no overt abnormalities compared to wild-type littermates. They are healthy, viable, and fertile, with normal growth, behavior, and reproductive performance. No breeding or welfare issues have been identified in animals carrying a single mutant allele, and the mutation does not impair colony management or stock maintenance. |
| Breeding history | Arhgap15+/- mice were backcrossed to C57BL/6J for 5 generations before generating homozygotes. Mice homozygous for the Arhgap15-targeted gene were viable, fertile, and displayed a normal life-span in a conventional mouse facility. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- The RacGAP ArhGAP15 is a master negative regulator of neutrophil functions.;Costa Carlotta, Germena Giulia, Martin-Conte Erica L, Molineris Ivan, Bosco Eleonora, Marengo Stefano, Azzolino Ornella, Altruda Fiorella, Ranieri V Marco, Hirsch Emilio, ;2011;Blood;118;1099-108; 21551229