C57BL/6-Tmem38aem1Gtu/Cnbc
| Status | Available to order |
| EMMA ID | EM:16206 |
| Citation information | RRID:IMSR_EM:16206 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Tmem38aem1Gtu/Cnbc |
| Alternative name | Tmem38atm1Gtu |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Tmem38aem1Gtu |
| Gene/Transgene symbol | Tmem38a |
Information from provider
| Provider | Ignacio Perez de Castro |
| Provider affiliation | Instituto de Salud Carlos III |
| Genetic information | Tmem38a targeted knock-in carrying the D260N (p.Asp260Asn) missense mutation. TMEM38A-D260N variant has been reported in Emery-Dreifuss muscular dystrophy patients (Meinke P. et al., EBioMedicine, 2020, 51:102587). |
| Phenotypic information | Homozygous:Homozygous mice are born at Mendelian rates. No survival, molecular or histological analysis has been carried out.Heterozygous:No survival, molecular or histological analysis has been carried out with heterozygous mice. |
| Breeding history | Hyperovulated C57BL/6J-enriched females were mated with C57BL/6J-enriched males to generate E0.5 embryos. Embryos were harvested from the females and electroporated with Cas9/sgRNA ribonucleoprotein complexes plus a single-stranded DNA template carrying the D260N mutation; 24 hours post-electroporation, embryos were transferred to pseudopregnant recipient females. Founder mice were screened by PCR and Sanger sequencing to identify D260N carriers, and all subsequent breeding was performed in a C57BL/6J-enriched background. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6J males |