C57BL/6N-Usp54tm1b(KOMP)Wtsi/H

Status

Only small colony available

EMMA IDEM:16266
Citation informationRRID:IMSR_EM:16266 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Usp54tm1b(KOMP)Wtsi/H
Alternative nameEPD0833_2_B10
Strain typeTargeted Mutant Strains
Allele/Transgene symbolUsp54tm1b(KOMP)Wtsi
Gene/Transgene symbolUsp54
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Mary Lyon Centre at MRC Harwell
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis mouse line originates from KOMP ES clone EPD0833_2_B10. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele. Click here for more information on KOMP final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)
  • increased neutrophil cell number / IMPC
  • enlarged heart / IMPC
  • decreased hemoglobin content / IMPC
  • increased circulating alanine transaminase level / IMPC
  • decreased lymphocyte cell number / IMPC
  • increased circulating aspartate transaminase level / IMPC
  • decreased circulating serum albumin level / IMPC
  • decreased circulating total protein level / IMPC
  • decreased circulating amylase level / IMPC
  • increased red blood cell distribution width / IMPC
  • decreased circulating fructosamine level / IMPC
IMPC phenotypes (gene matching)
  • decreased circulating amylase level / IMPC
  • decreased hemoglobin content / IMPC
  • enlarged heart / IMPC
  • decreased circulating total protein level / IMPC
  • increased red blood cell distribution width / IMPC
  • increased neutrophil cell number / IMPC
  • increased circulating aspartate transaminase level / IMPC
  • decreased circulating fructosamine level / IMPC
  • decreased circulating serum albumin level / IMPC
  • decreased lymphocyte cell number / IMPC
  • increased circulating alanine transaminase level / IMPC

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Order (limited)

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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