C57BL/6N-Nedd4tm2b(EUCOMM)Hmgu/H

Status

Only small colony available

EMMA IDEM:16326
Citation informationRRID:IMSR_EM:16326 

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International strain nameC57BL/6N-Nedd4tm2b(EUCOMM)Hmgu/H
Alternative nameHEPD0814_3_B10
Strain typeTargeted Mutant Strains
Allele/Transgene symbolNedd4tm2b(EUCOMM)Hmgu
Gene/Transgene symbolNedd4
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Mary Lyon Centre at MRC Harwell
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis mouse line originates from EUCOMM ES clone HEPD0814_3_B10. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele. Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)
  • increased leukocyte cell number / IMPC
  • tremors / IMPC
  • decreased locomotor activity / IMPC
  • abnormal gait / IMPC
  • abnormal behavior / IMPC
  • increased lymphocyte cell number / IMPC
  • impaired glucose tolerance / IMPC
  • decreased circulating creatinine level / IMPC
  • increased grip strength / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • decreased thigmotaxis / IMPC
IMPC phenotypes (gene matching)
  • decreased circulating creatinine level / IMPC
  • decreased thigmotaxis / IMPC
  • increased lymphocyte cell number / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • increased leukocyte cell number / IMPC
  • decreased locomotor activity / IMPC
  • tremors / IMPC
  • impaired glucose tolerance / IMPC
  • abnormal gait / IMPC
  • increased grip strength / IMPC
  • abnormal behavior / IMPC
MGI phenotypes (gene matching)
  • decreased body weight / MGI
  • abnormal T cell activation / MGI
  • hemorrhage / MGI
  • neonatal lethality / MGI
  • increased IgM level / MGI
  • abnormal neuron morphology / MGI
  • abnormal excitatory postsynaptic currents / MGI
  • nervous system phenotype / MGI
  • fetal growth retardation / MGI
  • abnormal miniature excitatory postsynaptic currents / MGI
  • abnormal synaptic vesicle number / MGI
  • increased T cell number / MGI
  • decreased B cell number / MGI
  • decreased T cell proliferation / MGI
  • increased CD4-positive, alpha beta T cell number / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • abnormal dendrite morphology / MGI
  • decreased IgG1 level / MGI
  • decreased IgG2b level / MGI
  • decreased IgG3 level / MGI
  • decreased interleukin-2 secretion / MGI
  • abnormal synapse morphology / MGI
  • perinatal lethality, complete penetrance / MGI
  • prenatal lethality, complete penetrance / MGI
  • lethality throughout fetal growth and development, incomplete penetrance / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

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