C57BL/6N-F2tm1b(EUCOMM)Hmgu/H

Status

Only small colony available

EMMA IDEM:16327
Citation informationRRID:IMSR_EM:16327 

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International strain nameC57BL/6N-F2tm1b(EUCOMM)Hmgu/H
Alternative nameHEPD0720_4_G11
Strain typeTargeted Mutant Strains
Allele/Transgene symbolF2tm1b(EUCOMM)Hmgu
Gene/Transgene symbolF2
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Mary Lyon Centre at MRC Harwell
Provider affiliationMary Lyon Centre at MRC Harwell
Genetic informationThis mouse line originates from EUCOMM ES clone HEPD0720_4_G11. For further details on the construction of this clone see the page at the IMPC portal. The critical exon(s) were flanked by loxP sites, and subsequent cre expression excised this critical sequence resulting in a knockout reporter allele. Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

IMPC phenotypes (allele matching)
  • tremors / IMPC
  • decreased exploration in new environment / IMPC
  • decreased mean corpuscular volume / IMPC
  • increased circulating serum albumin level / IMPC
  • decreased lean body mass / IMPC
  • increased total body fat amount / IMPC
  • increased circulating fructosamine level / IMPC
  • decreased bone mineral content / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
IMPC phenotypes (gene matching)
  • decreased bone mineral content / IMPC
  • increased total body fat amount / IMPC
  • decreased exploration in new environment / IMPC
  • increased circulating fructosamine level / IMPC
  • tremors / IMPC
  • decreased lean body mass / IMPC
  • decreased mean corpuscular volume / IMPC
  • preweaning lethality, incomplete penetrance / IMPC
  • increased circulating serum albumin level / IMPC
MGI phenotypes (gene matching)
  • distended pericardium / MGI
  • decreased embryo size / MGI
  • abnormal visceral yolk sac morphology / MGI
  • pale yolk sac / MGI
  • embryonic growth arrest / MGI
  • hemorrhage / MGI
  • intracranial hemorrhage / MGI
  • no abnormal phenotype detected / MGI
  • abnormal blood coagulation / MGI
  • abnormal vitelline vasculature morphology / MGI
  • decreased susceptibility to induced arthritis / MGI
  • pallor / MGI
  • hemopericardium / MGI
  • homeostasis/metabolism phenotype / MGI
  • abnormal circulating protein level / MGI
  • increased bleeding time / MGI
  • decreased susceptibility to endotoxin shock / MGI
  • decreased platelet aggregation / MGI
  • lethality, incomplete penetrance / MGI
  • postnatal lethality, complete penetrance / MGI
  • neonatal lethality, complete penetrance / MGI
  • embryonic lethality, incomplete penetrance / MGI
  • embryonic lethality during organogenesis, incomplete penetrance / MGI
  • lethality throughout fetal growth and development, incomplete penetrance / MGI
  • purpura / MGI
  • embryo tissue necrosis / MGI

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Example health report
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